Related Experiment Video
Updated: Jul 3, 2026
Peptide Scanning-assisted Identification of a Monoclonal Antibody-recognized Linear B-cell Epitope
Published on: March 24, 2017
[Preparation and identification of antibody against Cecropin-XJ]
Shu-Tao Zheng1, Zhong-Yuan Liu, Hui Wang
1Key Laboratory of Molecule Biology, College of Life Science and Technology, Xinjiang University, China. zst824@163.com
Aim:
To prepare the mouse antibody against Cecropin-XJ, identify its specificity and use it in cellular localization of Cecropin-XJ in vitro.
Methods:
The cDNA sequence of Cecropin-XJ with signal peptide sequence was subcloned into the eukaryotic expression vector pcDNA3. The recombinant plasmid was named pcDNA3-Cecropin-XJ, which was used as an antigen to immune the Kunming white mouse. Meanwhile, the cDNA sequence was subcloned into the fusion expression vector and the fusion protein was expressed as a test antigen.
Results:
Indirect ELISA showed that the fifth immunity's titer was highest. The immune gold-conjugated experiment showed that the prepared antibody was clearly and efficiently localized in prokaryotic cells where the Cecropin-XJ worked.
Conclusion:
The antibody against Cecropin-XJ has high immune reactivity and specificity, which is beneficial to further study on Cecropin-XJ function and antibody preparation of small peptides.
