Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: Jul 2, 2026

Single Droplet Digital Polymerase Chain Reaction for Comprehensive and Simultaneous Detection of Mutations in Hotspot Regions
08:23

Single Droplet Digital Polymerase Chain Reaction for Comprehensive and Simultaneous Detection of Mutations in Hotspot Regions

Published on: September 25, 2018

Multiplex detection of mutations.

David S Perlin1, Sergey Balashov, Steven Park

  • 1Public Health Research Institute, UMDNJ-New Jersey Medical School, International Center for Public Health, Newark, NJ, USA.

Methods in Molecular Biology (Clifton, N.J.)
|August 13, 2008
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Calcineurin-Dependent Stress Adaptation Enables Caspofungin Heteroresistance Leading to Stable Resistance in Candida Glabrata.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)·2026
Same author

Population genomics reveals a long-term increase in azole resistance associated with the emerging cluster AZR among <i>Candida tropicalis</i> causing invasive infections.

Emerging microbes & infections·2026
Same author

Facile generation of <i>Hermes</i> insertion mutants in prototrophic <i>Candida glabrata</i> for use in nutrient-limited environments.

Microbiology spectrum·2026
Same author

Multidimensional assessment of in-host fitness costs of echinocandin resistance in the opportunistic fungal pathogen <i>Candida glabrata</i> reveals the niche-specific requirement for <i>FKS1</i> and <i>FKS2</i> during infection and gut colonization.

Antimicrobial agents and chemotherapy·2026
Same author

Profound cell wall remodeling in <i>Candida parapsilosis</i> during systemic infection confers simultaneous tolerance to echinocandins and host immunity.

Microbiology spectrum·2026
Same author

Enhanced Fatigue Performance of VAR/EBR Nitinol for Transcatheter Heart Valve Applications: Material Characterization and Design Implications.

Journal of biomedical materials research. Part B, Applied biomaterials·2026
Same journal

Nanotechnology-Stem Cell Strategies in 3D Glioblastoma Organoid: Targeting Glioma Stem Cells Within a Complex Tumor Microenvironment.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Mapping the 3D Chromosome Organization of a Biosynthetic Gene Cluster by Capture Hi-C (CHi-C).

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Mapping the 3D Chromosome Organization of Streptomyces by Hi-C.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

CUT&Tag Epigenomic Profiling of Biosynthetic Gene Clusters in Arabidopsis thaliana.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Rhizobium rhizogenes-Mediated Hairy Root Transformation Protocol for Lotus japonicus and Other Legumes.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Characterization of Bioactive Saponins from Sea Cucumbers.

Methods in molecular biology (Clifton, N.J.)·2026
See all related articles

This study presents a multiplexed real-time PCR assay using Molecular Beacon probes for detecting mutations in the Aspergillus fumigatus CYP51A gene. This method enables rapid and reliable identification of triazole resistance mutations.

Area of Science:

  • Molecular biology
  • Medical diagnostics
  • Mycology

Background:

  • Accurate detection of genetic mutations is crucial for molecular diagnostics.
  • Distinguishing between wild-type and mutant alleles presents challenges in nucleic acid-based assays.
  • Real-time PCR with fluorescence probes offers a powerful tool for mutation detection.

Purpose of the Study:

  • To develop and apply a multiplexed real-time PCR assay for detecting mutations.
  • To identify specific mutations in codon 54 of the CYP51A gene in Aspergillus fumigatus.
  • To facilitate the detection of mutations conferring triazole resistance.

Main Methods:

  • Utilized multiplexed real-time polymerase chain reaction (PCR).
  • Employed Molecular Beacon probes for self-reporting fluorescence detection.

More Related Videos

Wild-type Blocking PCR Combined with Direct Sequencing as a Highly Sensitive Method for Detection of Low-Frequency Somatic Mutations
10:41

Wild-type Blocking PCR Combined with Direct Sequencing as a Highly Sensitive Method for Detection of Low-Frequency Somatic Mutations

Published on: March 29, 2017

Visual Detection of Multiple Nucleic Acids in a Capillary Array
08:56

Visual Detection of Multiple Nucleic Acids in a Capillary Array

Published on: November 15, 2017

Related Experiment Videos

Last Updated: Jul 2, 2026

Single Droplet Digital Polymerase Chain Reaction for Comprehensive and Simultaneous Detection of Mutations in Hotspot Regions
08:23

Single Droplet Digital Polymerase Chain Reaction for Comprehensive and Simultaneous Detection of Mutations in Hotspot Regions

Published on: September 25, 2018

Wild-type Blocking PCR Combined with Direct Sequencing as a Highly Sensitive Method for Detection of Low-Frequency Somatic Mutations
10:41

Wild-type Blocking PCR Combined with Direct Sequencing as a Highly Sensitive Method for Detection of Low-Frequency Somatic Mutations

Published on: March 29, 2017

Visual Detection of Multiple Nucleic Acids in a Capillary Array
08:56

Visual Detection of Multiple Nucleic Acids in a Capillary Array

Published on: November 15, 2017

  • Focused on analyzing codon 54 of the CYP51A gene.

    • Main Results:

    • Successfully applied the assay to detect mutations in the target gene.
    • Demonstrated the capability for high-throughput screening of multiple mutations.
    • The assay proved effective in identifying mutations associated with triazole resistance.

    Conclusions:

    • The developed multiplexed real-time PCR assay is effective for detecting CYP51A gene mutations.
    • This method provides a rapid, reliable, and cost-effective approach for identifying triazole resistance in Aspergillus fumigatus.
    • The assay supports high-throughput screening for multiple mutations, enhancing diagnostic capabilities.