Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

The Eukaryotic Promoter Region02:40

The Eukaryotic Promoter Region

The eukaryotic promoter region is a segment of DNA located upstream of a gene. It contains an RNA polymerase binding site, a transcription start site, and several cis-regulatory sequences.  The proximal promoter region is located in the vicinity of the gene and has cis-regulatory sequences and the core promoter. The core promoter is the binding site for RNA polymerase and is usually located between -35 and +35 nucleotides from the transcription start site. The distal promoter regions are...
The Eukaryotic Promoter Region02:40

The Eukaryotic Promoter Region

The eukaryotic promoter region is a segment of DNA located upstream of a gene. It contains an RNA polymerase binding site, a transcription start site, and several cis-regulatory sequences.  The proximal promoter region is located in the vicinity of the gene and has cis-regulatory sequences and the core promoter. The core promoter is the binding site for RNA polymerase and is usually located between -35 and +35 nucleotides from the transcription start site. The distal promoter regions are...
Reporter Genes02:11

Reporter Genes

Reporter genes are a type of protein-coding gene that are often tagged to a gene of interest. Once inside a target cell, reporter genes usually produce visually identifiable characteristics like fluorescence and luminescence when expressed along with the gene of interest. Thus, reporter genes “report” the presence or absence of genes of interest in an organism, determine the gene expression pattern, or track the physical location of a DNA segment or protein in the cell.
Commonly used reporter...
Cell Specific Gene Expression01:58

Cell Specific Gene Expression

Multicellular organisms contain a variety of structurally and functionally distinct cell types, but the DNA in all the cells originated from the same parent cells. The differences in the cells can be attributed to the differential gene expression. Liver cells, whose functions include detoxification of blood, production of bile to metabolize fats, and synthesis of proteins essential for metabolism, must express a specific set of genes to perform their functions. Gene expression also varies with...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

ODAPH (p.Arg77*) Phenotype and Onset of Ameloblast Pathology During Postsecretory Transition Demonstrated by FIB-SEM Analyses of Odaph<sup>C41*/C41*</sup> Mice.

Calcified tissue international·2026
Same author

Hereditary enamel defects with comorbidities.

Journal of the American Dental Association (1939)·2026
Same author

Novel <i>ITGB6</i> Mutations Causing Amelogenesis Imperfecta.

Genes·2026
Same author

Generation and characterization of a murine amelogenesis imperfecta model.

Archives of oral biology·2026
Same author

Novel <i>MMP20</i> (matrix metalloproteinase 20) mutations causing hypoplastic-hypomaturation amelogenesis imperfecta.

Journal of dental sciences·2026
Same author

Citrate Transporter Expression and Localization: The <i>Slc13a5</i><sup>Flag</sup> Mouse Model.

International journal of molecular sciences·2025
Same journal

Enhancing the culture of mouse primary fibroblasts to study age-dependent effects in skin tissue engineering in vitro.

Cells, tissues, organs·2026
Same journal

Role of the Proteasome System in Shaping Cellular Immunological Characteristics and Its Impact in Modulating the Pathogenesis of Immune-Related Diseases.

Cells, tissues, organs·2026
Same journal

The Impact of Biomaterial Charge on Cells' Bioelectrical Signaling.

Cells, tissues, organs·2026
Same journal

Homer 2 regulates muscle differentiation with NFATc1.

Cells, tissues, organs·2026
Same journal

Integrating Cells, Biomaterials, and Advanced Engineering for Next-Generation Peripheral Nerve Repair.

Cells, tissues, organs·2026
Same journal

Rapid, Growth Factor-Reduced Induction of Functional Neurons from hiPSCs.

Cells, tissues, organs·2026
See all related articles

Related Experiment Video

Updated: Jul 2, 2026

A Web-Based Workflow for Selecting Gene- and Tissue-Specific Enhancers
08:12

A Web-Based Workflow for Selecting Gene- and Tissue-Specific Enhancers

Published on: July 18, 2025

Identifying promoter elements necessary for enamelin tissue-specific expression.

Petros Papagerakis1, Yuanyuan Hu, Ling Ye

  • 1Department of Orthodontics and Pediatric Dentistry, University of Michigan School of Dentistry, Ann Arbor, Mich. 48108, USA. petrosp@umich.edu

Cells, Tissues, Organs
|August 16, 2008
PubMed
Summary
This summary is machine-generated.

Researchers identified key DNA regions controlling enamelin (Enam) gene expression during tooth development. A 5.2 kb DNA fragment is sufficient to ensure Enam is correctly expressed only in ameloblasts, crucial for enamel formation.

More Related Videos

Prediction and Validation of Gene Regulatory Elements Activated During Retinoic Acid Induced Embryonic Stem Cell Differentiation
09:07

Prediction and Validation of Gene Regulatory Elements Activated During Retinoic Acid Induced Embryonic Stem Cell Differentiation

Published on: June 21, 2016

An Integrated Workflow to Study the Promoter-Centric Spatio-Temporal Genome Architecture in Scarce Cell Populations
11:36

An Integrated Workflow to Study the Promoter-Centric Spatio-Temporal Genome Architecture in Scarce Cell Populations

Published on: April 21, 2023

Related Experiment Videos

Last Updated: Jul 2, 2026

A Web-Based Workflow for Selecting Gene- and Tissue-Specific Enhancers
08:12

A Web-Based Workflow for Selecting Gene- and Tissue-Specific Enhancers

Published on: July 18, 2025

Prediction and Validation of Gene Regulatory Elements Activated During Retinoic Acid Induced Embryonic Stem Cell Differentiation
09:07

Prediction and Validation of Gene Regulatory Elements Activated During Retinoic Acid Induced Embryonic Stem Cell Differentiation

Published on: June 21, 2016

An Integrated Workflow to Study the Promoter-Centric Spatio-Temporal Genome Architecture in Scarce Cell Populations
11:36

An Integrated Workflow to Study the Promoter-Centric Spatio-Temporal Genome Architecture in Scarce Cell Populations

Published on: April 21, 2023

Area of Science:

  • Developmental Biology
  • Molecular Genetics
  • Biochemistry

Background:

  • Enamel formation (amelogenesis) relies on precise gene expression timing.
  • Mechanisms controlling enamel matrix protein gene transcription initiation and termination are poorly understood.
  • Enamelin (Enam) is a critical enamel matrix protein.

Purpose of the Study:

  • To identify cis-regulatory regions essential for normal enamelin (Enam) gene expression.
  • To elucidate the molecular control of Enam cell- and stage-specific expression during amelogenesis.

Main Methods:

  • Sequence analysis of the Enam promoter 5'-noncoding region to identify potential cis-regulatory elements.
  • Generation of transgenic mice using DNA constructs linking 5.2 kb or 3.9 kb of the Enam promoter to an LacZ reporter gene.
  • Analysis of LacZ expression patterns in transgenic mice to assess Enam regulatory regions.

Main Results:

  • A 3.9 kb Enam promoter fragment linked to LacZ showed no expression in ameloblasts but ectopic expression in osteoblasts.
  • A 5.2 kb Enam promoter fragment linked to LacZ successfully recapitulated the endogenous Enam ameloblast-specific expression pattern.
  • Specific cis-regulatory elements within the 5.2 kb region are necessary for correct Enam expression.

Conclusions:

  • The 5.2 kb upstream region of the Enam gene contains critical cis-regulatory elements for ameloblast-specific expression.
  • These findings provide new insights into the molecular mechanisms regulating enamelin gene transcription during tooth development.
  • Understanding these regulatory regions is key to comprehending the precise control of amelogenesis.