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Preparation of Naringenin Solution for In Vivo Application
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Preparation of Naringenin Solution for In Vivo Application

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Naringenin inhibits human osteoclastogenesis and osteoclastic bone resorption

V D La1, S Tanabe, D Grenier

  • 1Groupe de Recherche en Ecologie Buccale, Faculté de Médecine Dentaire, Université Laval, 2420 Rue de la Terrasse, Quebec City, Quebec, Canada.

Journal of Periodontal Research
|August 19, 2008
PubMed
Abstract

Insights

Naringenin significantly inhibits human osteoclastogenesis and bone resorption by decreasing key inflammatory factors. This flavonoid shows potential for treating bone diseases like periodontitis.

Area of Science:

  • Biochemistry
  • Cell Biology
  • Pharmacology

Background:

  • Naringenin is a flavonoid with diverse pharmacological effects.
  • Osteoclastogenesis and bone resorption are critical processes in bone metabolism.
  • Dysregulation of these processes contributes to various bone diseases.

Purpose of the Study:

  • To investigate the impact of naringenin on human osteoclastogenesis.
  • To evaluate naringenin's effect on osteoclastic bone resorption.

Main Methods:

  • Human osteoclast precursor cells were cultured and stimulated with RANKL and M-CSF.
  • Osteoclastogenesis was quantified by TRAP staining.
  • Bone resorption was assessed using an OsteoAssay human bone plate.
  • Secretion of inflammatory cytokines (IL-1α, IL-23, MCP-1) was measured via ELISA.

Main Results:

  • Naringenin demonstrated no toxicity at concentrations up to 50 µg/ml.
  • Naringenin significantly inhibited osteoclastogenesis in a dose-dependent manner (up to 96% inhibition).
  • Naringenin markedly reduced the secretion of IL-1α, IL-23, and MCP-1.
  • Naringenin significantly decreased bone resorption markers (up to 86% reduction).

Conclusions:

  • Naringenin effectively inhibits human osteoclastogenesis and osteoclastic bone resorption.
  • Naringenin's anti-resorptive properties suggest its potential as a therapeutic agent.
  • Naringenin may be beneficial in managing bone-related conditions, including periodontitis.

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