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Updated: Jul 2, 2026

Preparation of Fungal and Plant Materials for Structural Elucidation Using Dynamic Nuclear Polarization Solid-State NMR
Published on: February 12, 2019
High-resolution 14N-edited 1H-13C correlation NMR experiment to study biological solids
Jean-Paul Amoureux1, Julien Trébosc, Bingwen Hu
1Unité de Catalyse et Chimie du Solide, UMR-CNRS 8181, Université de Lille 1, 59652 Villeneuve d'Ascq cedex, France. jean-paul.amoureux@univ-lille1.fr
Abstract:
It was recently shown that nuclear magnetic resonance (NMR) spectra of nitrogen-14 (spin I=1) can be obtained by indirect detection via spin S=1/2 nuclei in powders spinning at the magic angle. An increased number of solid-state NMR methods are now available to tailor sequences for specific purposes, e.g., hetero-nuclear dipolar recoupling or homo-nuclear dipolar decoupling schemes. Here, we combine the latest recoupling and decoupling techniques to obtain high-resolution (1)H-(13)C through-space correlation spectra, where only the correlation peaks of those carbons close to nitrogen nuclei are observed. The experiment is demonstrated on a (13)C enriched l-histidine. HCl x H(2)O powder sample.
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