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TiO2 nanowire bundle microelectrode based impedance immunosensor for rapid and sensitive detection of Listeria
Ronghui Wang1, Wenjun Dong, Chuanmin Ruan
1Department of Biological and Agricultural Engineering, Cell and Molecular Biology Program, Center of Excellence for Poultry Science, University of Arkansas, Fayetteville, Arkansas 72701, USA.
Abstract:
A novel TiO 2 nanowire bundle microelectrode based immunosensor was demonstrated as a more sensitive, specific, and rapid technology for detection of Listeria monocytogenes. TiO 2 nanowire bundle was prepared through a hydrothermal reaction of alkali with TiO 2 powder and connected to gold microelectrodes with mask welding. Monoclonal antibodies were immobilized on the surface of a TiO 2 nanowire bundle to specifically capture L. monocytogenes. Impedance change caused by the nanowire-antibody-bacteria complex was measured and correlated to bacterial number. This nanowire bundle based immunosensor could detect as low as 10 (2) cfu/ml of L. monocytogenes in 1 h without significant interference from other foodborne pathogens.
