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[Experiences with isolated organ studies on pathological human arteries]
Z Szombathelyi1, E Kárpáti, A Till
1Köbányai Gyógyszerárugyár, Farmakológiai Kutató Központ, Budapest.
Insights
Human arteries from lower limb amputations, despite disease, retain some contractile function. Arterial rings responded to KCl, serotonin, and prostaglandin F2 alpha, but not phenylephrine.
Area of Science:
- Vascular biology
- Human physiology
- Pharmacology
Context:
- Pathological arteries from lower limb amputations due to arteriosclerosis obliterans (ASO), thromboangiitis obliterans (TAO), and diabetes mellitus (DIA) were analyzed.
- The study investigated the functional integrity of isolated human arteries under various pathological conditions.
Purpose:
- To assess the contractile responses of human arteries to different agonists (KCl, serotonin, prostaglandin F2 alpha, phenylephrine).
- To determine how disease state, patient age, artery location, and hypertension affect arterial contractility.
Summary:
- Most arterial rings exhibited contraction to KCl, serotonin, and prostaglandin F2 alpha, but 66% showed no response to phenylephrine.
- Contractile force varied significantly based on diagnosis (TAO > ASO > DIA), patient age, anatomical location (TAO), and hypertension status (ASO).
Impact:
- Despite advanced disease stages leading to amputation, human arteries maintain partial functional integrity.
- Findings provide insights into vascular dysfunction in peripheral artery diseases and diabetes, informing potential therapeutic strategies.
Abstract:
Pathological, muscular arteries (common and superficial femoral [FC, FS], anterior and posterior tibial [TA, TP] arteries) of patients suffering from arteriosclerosis obliterans (ASO), thromboangiitis obliterans (TAO), and diabetes mellitus (DIA), removed during amputation of the lower limb were studied as isolated organs. The vessels were cut into transverse rings and contractile force was measured isometrically. The total number of used rings was 828. The following agonists were applied: KCl (80 mM), serotonin (5-HT) (10 microM), prostaglandin F2 alpha (PGF2 alpha) (0.1 mM) or phenylephrine (PE) (10 microM). It was established that applying KCl, 5-HT or PGF2 alpha, the majority of arterial rings display a contraction, but most of the preparations (66%) give no response against PE. The measure of contraction depends on the diagnosis (TAO greater than ASO greater than DIA), on the age of patient and also the anatomical location of the artery in the case of TAO (TP greater than greater than TA), on the associated hypertension in the case of ASO (normotensive greater than hypertensive) and finally on the time elapsed between the operation and usage of preparation if the agonist is KCl. As a conclusion, despite the terminal clinical stage the majority of studied human arteries retained at least a part of their functional integrity.