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Related Concept Videos

Capillary Electrophoresis: Instrumentation01:20

Capillary Electrophoresis: Instrumentation

Capillary electrophoresis instrumentation typically consists of several key components. A high-voltage power supply generates the electric field necessary for the separation by connecting to an anode (the positively charged electrode) and a cathode (the negatively charged electrode) located in buffer reservoirs at each end of the capillary tube. The system includes a sample vial, a fused silica capillary tube coated with polyimide for mechanical strength through which the sample components...
Electrophoresis: Overview01:20

Electrophoresis: Overview

Electrophoresis is a powerful analytical separation technique that relies on the differential migration of charged species when subjected to an electric field. The core strength of electrophoresis lies in its ability to separate high-molecular-weight species in complex mixtures. It has found widespread use in biochemistry, molecular biology, and analytical chemistry, allowing the separation of compounds like amino acids, nucleotides, carbohydrates, and proteins with excellent resolution.
There...
Capillary Electrophoresis: Applications01:30

Capillary Electrophoresis: Applications

Capillary electrophoretic separations offer various modes, each with unique applications. These modes include capillary zone electrophoresis, capillary gel electrophoresis, capillary array electrophoresis, capillary isoelectric focusing, capillary isotachophoresis, micellar electrokinetic chromatography, and capillary electrochromatography.
Capillary zone electrophoresis (CZE) separates ionic components based on their electrophoretic mobility. It has been used to separate proteins, amino acids,...
High-Performance Liquid Chromatography: Elution Process01:05

High-Performance Liquid Chromatography: Elution Process

In High-Performance Liquid Chromatography (HPLC), the elution process is critical to the separation of analytes and the quality of chromatographic results. Elution describes how compounds move through the column and separate based on their interactions with the mobile and stationary phases. This process determines the resolution, peak shape, and retention times in the chromatogram, which are essential for identifying and quantifying components in complex mixtures. Understanding the elution...
Ion-Exchange Chromatography01:09

Ion-Exchange Chromatography

Ion-exchange chromatography, or IEC, is a technique for separating ions based on their affinity for the stationary phase. The stationary phase is a cross-linked polymer resin with covalently attached ionic functional groups. The functional groups can be either positively charged (cation exchangers) or negatively charged (anion exchangers). A cation exchanger consists of a polymeric anion and active cations, while an anion exchanger is a polymeric cation with active anions. The choice of...
Gas Chromatography: Types of Columns and Stationary Phases01:17

Gas Chromatography: Types of Columns and Stationary Phases

Gas chromatography (GC) relies on stationary phases to separate and analyze components in a sample. There are two main types of stationary phases: liquid and solid. Liquid stationary phases are non-volatile, thermally stable, and chemically inert liquids coated onto the column. Solid stationary phases are particles of adsorbent material, such as silica gel or molecular sieves.
For an analyte to remain on the column for a sufficient amount of time, it must exhibit some level of compatibility (or...

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Large-scale Top-down Proteomics Using Capillary Zone Electrophoresis Tandem Mass Spectrometry
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Published on: October 24, 2018

Modeling wall effects in capillary electrochromatography.

N Scales1, R N Tait

  • 1Department of Electronics, Carleton University, 1125 Colonel By Drive, Ottawa, Ontario K1S 5B6, Canada.

Journal of Chromatography. A
|August 30, 2008
PubMed
Summary
This summary is machine-generated.

A new volume averaging model accurately simulates fluid flow in packed microchannels for capillary electrochromatography. It accounts for porosity variations and zeta potential mismatches, matching experimental results.

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Area of Science:

  • Analytical Chemistry
  • Chemical Engineering
  • Fluid Dynamics

Background:

  • Microchannel fluid dynamics are crucial for separation sciences.
  • Modeling flow in porous media, like packed columns, presents challenges.
  • Electroosmotic and pressure-driven flows require distinct modeling approaches.

Purpose of the Study:

  • To develop and validate a volume averaging technique for modeling flow in microchannels.
  • To apply this model to packed capillary electrochromatography (CEC) columns.
  • To investigate the impact of porosity variations and zeta potential mismatches on flow behavior.

Main Methods:

  • Utilized a volume averaging technique to model fluid flow.
  • Applied the model to packed capillary electrochromatography columns.
  • Compared numerical simulations with existing experimental data.

Main Results:

  • The model successfully simulates both electroosmotic and pressure-driven flow.
  • It accurately accounts for porosity variations and zeta potential mismatches.
  • Simulated concentration profiles closely matched experimental observations, including wall effects.

Conclusions:

  • The volume averaging technique provides a robust method for modeling flow in packed microchannels.
  • The model's ability to reproduce experimental wall effects validates its effectiveness for CEC applications.
  • This technique is applicable to both porous and open channel systems.