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A microprocessor-regulated constant voltage, current, wattage, and temperature electrophoresis power supply
D W Mincey1, K J Kuzior, L H Allen
1Department of Chemistry, Youngstown State University, Ohio 44555.
Analytical Biochemistry
|March 2, 1991
Summary
A digital microcomputer replaced analog circuitry in electrophoresis power supplies, enhancing stability and versatility. This innovation allows for precise control of voltage, current, and other parameters, improving experimental outcomes.
Area of Science:
- Biotechnology
- Electrical Engineering
- Analytical Chemistry
Background:
- Traditional electrophoresis power supplies rely on analog control circuitry.
- Analog systems can be limited in precision and versatility for advanced electrophoresis techniques.
Purpose of the Study:
- To develop a more stable and versatile electrophoresis power supply.
- To integrate digital microcomputer control for enhanced performance.
Main Methods:
- Replaced analog control with a digital microcomputer system.
- Utilized analog-to-digital converters for real-time monitoring of voltage and current.
- Employed microcomputer programming for parameter comparison and output voltage calculation.
- Implemented digital-to-analog converters for precise voltage control.
- Developed BASIC programming subroutines for maintaining constant power parameters.
Main Results:
- Achieved greater stability and versatility compared to conventional power supplies.
- Enabled easy integration of advanced features like automated endpoint detection and pulsed waveforms.
- Demonstrated precise control over voltage, current, power, and temperature at a rate of at least 20 times per second.
Conclusions:
- Microcomputer-based feedback systems offer superior performance for electrophoresis power supplies.
- The digital approach enhances experimental reproducibility and allows for sophisticated experimental designs.