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Updated: Jul 2, 2026

Digital Inline Holographic Microscopy (DIHM) of Weakly-scattering Subjects
Published on: February 8, 2014
Laplacian differential reconstruction of in-line holograms recorded at two different distances
Cheng-Shan Guo1, Qing-Yang Yue, Gong-Xiang Wei
1Department of Physics, Shandong Normal University, Jinan, China. guochsh@sdnu.edu.cn
Abstract:
We analyze the diffraction of the picture obtained by the subtraction of two in-line holograms recorded in two planes at distances of z and z+Deltaz apart from the object plane. Our theoretical analysis reveals that the reconstructed field at the object plane is approximately equal to the Laplacian second-order differentiation of the object wave in transverse direction when Deltaz is much smaller than the distance z, that is, the reconstructed image presents a high quality of edge enhancement. We further investigate the dependence of the edge-enhancement quality on the longitudinal differential distance Deltaz and find that the reconstructed images have the sharpest edge-enhancement and high signal-to-noise ratio at the same time only when the value of Deltaz/z lies between 0.7% and 0.9% under our experimental condition. We also construct a criteria function, named the entropy of the image, to automatically focus the edge-enhanced image and demonstrate its adaptability in experiments.

