Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Three-Dimensional Microscopy in Microbiology01:28

Three-Dimensional Microscopy in Microbiology

3
Three-dimensional imaging techniques are essential in cell biology, allowing researchers to visualize intricate cellular structures with high resolution. Two prominent methods, Differential Interference Contrast Microscopy (DIC) and Confocal Scanning Laser Microscopy (CSLM), provide distinct advantages for imaging live and thick specimens, respectively.Differential Interference Contrast MicroscopyDIC microscopy enhances contrast in transparent, unstained samples by converting phase...
3
Electron Microscope Tomography and Single-particle Reconstruction01:07

Electron Microscope Tomography and Single-particle Reconstruction

2.4K
Transmission electron microscopy (TEM) can be used to determine the 3D structure of biological samples with the help of techniques such as electron microscope tomography and single-particle reconstruction. While single-particle reconstruction can examine macromolecules and macromolecular complexes in vitro conditions only, tomography permits the study of cell components or small cells in vivo.
Electron Tomography
Electron tomography can be performed either in TEM or STEM (scanning transmission...
2.4K
Overview of Microscopy Techniques01:22

Overview of Microscopy Techniques

9.7K
The early pioneers of microscopy opened a window into the invisible world of microorganisms. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes that leveraged nonvisible light, such as fluorescence microscopy that uses an ultraviolet light source and electron microscopy that uses short-wavelength electron beams. These advances significantly improved magnification, image resolution, and contrast. By comparison, the...
9.7K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Altered structural connectivity in white matter lesion-induced mild cognitive impairment: a probabilistic fiber tracking and graph theory analysis.

Frontiers in neural circuits·2026
Same author

Sustained regression of a tislelizumab-reactivated tuberculous focus after short-course therapy: a case report.

BMC infectious diseases·2026
Same author

Single-exposure multi-wavelength optical diffraction tomography based on space-angle dual multiplexing holography.

Optics letters·2024
Same author

Simple system for realizing single-shot ultrafast sequential imaging based on spatial multiplexing in-line holography.

Optics express·2022
Same author

Hope During the COVID-19 Epidemic Decreased Anxiety and Depression Symptoms Mediated by Perceived Stress: Coping Style Differences Among Patients with COVID-19.

Current medical science·2022
Same author

Association between Changes in White Matter Microstructure and Cognitive Impairment in White Matter Lesions.

Brain sciences·2022

Related Experiment Video

Updated: Jun 6, 2025

Lensfree On-chip Tomographic Microscopy Employing Multi-angle Illumination and Pixel Super-resolution
08:41

Lensfree On-chip Tomographic Microscopy Employing Multi-angle Illumination and Pixel Super-resolution

Published on: August 16, 2012

11.5K

Portable single-shot lens-free tomographic microscope for imaging dynamic specimens.

Zhen-Wei Qin, Yang Yang, Yan-Ling Ma

    Optics Express
    |November 22, 2024
    PubMed
    Summary
    This summary is machine-generated.

    This study introduces a portable, single-shot lens-free microscope for 3D imaging of dynamic specimens using spatial multiplexing digital holography. The system achieves high-resolution 3D reconstructions from a single exposure, ideal for live or fast-moving samples.

    More Related Videos

    Lens-free Video Microscopy for the Dynamic and Quantitative Analysis of Adherent Cell Culture
    09:04

    Lens-free Video Microscopy for the Dynamic and Quantitative Analysis of Adherent Cell Culture

    Published on: February 23, 2018

    9.4K
    Dynamic Pore-scale Reservoir-condition Imaging of Reaction in Carbonates Using Synchrotron Fast Tomography
    10:18

    Dynamic Pore-scale Reservoir-condition Imaging of Reaction in Carbonates Using Synchrotron Fast Tomography

    Published on: February 21, 2017

    8.5K

    Related Experiment Videos

    Last Updated: Jun 6, 2025

    Lensfree On-chip Tomographic Microscopy Employing Multi-angle Illumination and Pixel Super-resolution
    08:41

    Lensfree On-chip Tomographic Microscopy Employing Multi-angle Illumination and Pixel Super-resolution

    Published on: August 16, 2012

    11.5K
    Lens-free Video Microscopy for the Dynamic and Quantitative Analysis of Adherent Cell Culture
    09:04

    Lens-free Video Microscopy for the Dynamic and Quantitative Analysis of Adherent Cell Culture

    Published on: February 23, 2018

    9.4K
    Dynamic Pore-scale Reservoir-condition Imaging of Reaction in Carbonates Using Synchrotron Fast Tomography
    10:18

    Dynamic Pore-scale Reservoir-condition Imaging of Reaction in Carbonates Using Synchrotron Fast Tomography

    Published on: February 21, 2017

    8.5K

    Area of Science:

    • Microscopy
    • Optical Imaging
    • Holography

    Background:

    • Traditional microscopy techniques often struggle with imaging dynamic or moving specimens in three dimensions.
    • Lens-free microscopy offers a simplified optical setup but can face challenges in achieving high resolution and depth information.

    Purpose of the Study:

    • To develop a portable, single-shot, lens-free tomographic microscope for high-resolution 3D imaging of dynamic biological samples.
    • To demonstrate the capability of spatial multiplexing in-line digital holography for capturing multiple perspectives in a single exposure.

    Main Methods:

    • A simplified system comprising a laser diode array, rectangular aperture, and CMOS image sensor with a global shutter was employed.
    • Spatial multiplexing in-line digital holography enabled simultaneous recording of holographic data from multiple illumination angles without mechanical scanning.
    • A novel data processing algorithm was developed for reconstructing 3D tomograms from the multiplexed hologram.

    Main Results:

    • High-quality 3D tomograms were reconstructed with a lateral resolution of 3.36μm and axial resolution of 6.39μm.
    • The system achieved a field-of-view of 5.06 mm² in a single camera exposure.
    • Successful 3D imaging of free-swimming paramecium in water demonstrated the system's capability for dynamic specimens.

    Conclusions:

    • The developed portable lens-free tomographic microscope is effective for 3D imaging of living or rapidly moving specimens.
    • The system shows significant potential for applications in resource-limited environments requiring rapid, high-resolution 3D imaging.
    • Spatial multiplexing digital holography offers a viable approach for single-shot, multi-angle data acquisition in microscopy.