Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Confocal Fluorescence Microscopy01:16

Confocal Fluorescence Microscopy

13.5K
Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...
13.5K
Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

7.1K
Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...
7.1K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Portable single-shot lens-free tomographic microscope for imaging dynamic specimens.

Optics express·2024
Same author

Single-exposure multi-wavelength optical diffraction tomography based on space-angle dual multiplexing holography.

Optics letters·2024
Same author

Cylindrical wave-based off-axis digital holography with long field of view.

Optics letters·2022
Same author

Polarization holographic microscope slide for birefringence imaging of anisotropic samples in microfluidics.

Optics express·2020
Same author

Single-shot ultrafast sequential holographic imaging with high temporal resolution and a large field of view.

Optics letters·2019
Same author

Single-shot quantitative birefringence microscopy for imaging birefringence parameters.

Optics letters·2019

Related Experiment Video

Updated: Aug 22, 2025

Evaluation and Manipulation of Neural Activity Using Two-Photon Holographic Microscopy
10:09

Evaluation and Manipulation of Neural Activity Using Two-Photon Holographic Microscopy

Published on: September 16, 2022

2.7K

Simple system for realizing single-shot ultrafast sequential imaging based on spatial multiplexing in-line

Hong-Yi Huang, Cheng-Shan Guo

    Optics Express
    |November 11, 2022
    PubMed
    Summary
    This summary is machine-generated.

    This study introduces a novel single-shot ultrafast sequential imaging system using spatial multiplexing in-line holography. The system achieves an unprecedented 7.5 trillion frames per second for studying dynamic processes.

    More Related Videos

    Time Multiplexing Super Resolving Technique for Imaging from a Moving Platform
    06:25

    Time Multiplexing Super Resolving Technique for Imaging from a Moving Platform

    Published on: February 12, 2014

    8.5K
    Compact Lens-less Digital Holographic Microscope for MEMS Inspection and Characterization
    10:28

    Compact Lens-less Digital Holographic Microscope for MEMS Inspection and Characterization

    Published on: July 5, 2016

    10.4K

    Related Experiment Videos

    Last Updated: Aug 22, 2025

    Evaluation and Manipulation of Neural Activity Using Two-Photon Holographic Microscopy
    10:09

    Evaluation and Manipulation of Neural Activity Using Two-Photon Holographic Microscopy

    Published on: September 16, 2022

    2.7K
    Time Multiplexing Super Resolving Technique for Imaging from a Moving Platform
    06:25

    Time Multiplexing Super Resolving Technique for Imaging from a Moving Platform

    Published on: February 12, 2014

    8.5K
    Compact Lens-less Digital Holographic Microscope for MEMS Inspection and Characterization
    10:28

    Compact Lens-less Digital Holographic Microscope for MEMS Inspection and Characterization

    Published on: July 5, 2016

    10.4K

    Area of Science:

    • Optics and Photonics
    • Ultrafast Imaging
    • Holography

    Background:

    • Studying ultrafast dynamic processes requires high-speed imaging techniques.
    • Traditional methods often face limitations in temporal resolution and complexity.

    Purpose of the Study:

    • To develop a simple yet powerful system for single-shot ultrafast sequential imaging.
    • To achieve extremely high frame rates for capturing transient phenomena.

    Main Methods:

    • Utilizing spatial multiplexing in-line holography.
    • Combining a mini-reflector delay-line array with digital in-line holography.
    • Generating independent probe sub-pulses for sequential image acquisition.

    Main Results:

    • Demonstrated a single-shot system capable of capturing multiple holographic frames.
    • Achieved a record frame rate of 7.5 trillion frames per second (Tfps).
    • Successfully imaged laser-induced air plasma dynamics.

    Conclusions:

    • The proposed system offers a simplified approach to ultrafast sequential imaging.
    • The high temporal resolution enables the study of femtosecond-order dynamic processes.
    • This technique has significant potential for scientific research in various fields.