Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Phase Contrast and Differential Interference Contrast Microscopy

Phase Contrast and Differential Interference Contrast Microscopy

Phase-Contrast Microscopes
In-phase-contrast microscopes, interference between light directly passing through a cell and light refracted by cellular components is used to create high-contrast, high-resolution images without staining. It is the oldest and simplest type of microscope that creates an image by altering the wavelengths of light rays passing through the specimen. Altered wavelength paths are created using an annular stop in the condenser. The annular stop produces a hollow cone of...

Electron Microscope Tomography and Single-particle Reconstruction

Electron Microscope Tomography and Single-particle Reconstruction

Transmission electron microscopy (TEM) can be used to determine the 3D structure of biological samples with the help of techniques such as electron microscope tomography and single-particle reconstruction. While single-particle reconstruction can examine macromolecules and macromolecular complexes in vitro conditions only, tomography permits the study of cell components or small cells in vivo.
Electron Tomography
Electron tomography can be performed either in TEM or STEM (scanning transmission...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Intergenerational effects of the microbiota on neurodevelopment: mechanisms and therapeutic perspectives.

Acta pharmacologica Sinica·2026

Same author

Portable single-shot lens-free tomographic microscope for imaging dynamic specimens.

Optics express·2024

Same author

Simple system for realizing single-shot ultrafast sequential imaging based on spatial multiplexing in-line holography.

Optics express·2022

Same author

SDF-1 expression and tumor-infiltrating lymphocytes identify clinical subtypes of triple-negative breast cancer with different responses to neoadjuvant chemotherapy and survival.

Frontiers in immunology·2022

Same author

Linking oxidative DNA lesion 8-OxoG to tumor development and progression.

Yi chuan = Hereditas·2022

Same author

Prevalence and clinical characteristics of COVID-19 in inpatients with schizophrenia in Wuhan, China.

World journal of psychiatry·2022

Same journal

Gaussian-modulated continuous-variable quantum key distribution over 60 km fiber using an integrated silicon photonic receiver.

Optics letters·2026

Same journal

E2E-OCT: end-to-end joint learning model using optical coherence tomography images for vocal cord leukoplakia diagnosis.

Optics letters·2026

Same journal

Holographic generation of panoramic 3D scenes by concave ellipsoidal mirror reflection.

Optics letters·2026

Same journal

Dual-pilot phase recovery with pair-wise maximum-ratio combining for coherent PONs.

Optics letters·2026

Same journal

Mapping the whispering gallery modes of a CaF<sub>2</sub> disk resonator with half-tapered fibers to estimate the fundamental mode volume.

Optics letters·2026

Same journal

Quantitative estimation of deep-subwavelength scale via dark-field scattering axial energy concentration decay profiles.

Optics letters·2026

See all related articles

Search research articles

Related Experiment Video

Updated: Apr 10, 2026

Lensfree On-chip Tomographic Microscopy Employing Multi-angle Illumination and Pixel Super-resolution

Lensfree On-chip Tomographic Microscopy Employing Multi-angle Illumination and Pixel Super-resolution

Published on: August 16, 2012

Single-exposure multi-wavelength optical diffraction tomography based on space-angle dual multiplexing holography.

Hong-Yi Huang, Qing-Yang Yue, Yang Yang

|June 2, 2024

Summary

This summary is machine-generated.

We developed a novel holographic system for fast, single-exposure multi-wavelength optical diffraction tomographic (ODT) imaging. This method reconstructs 3D refractive index tomograms without scanning, advancing biological and material science imaging.

More Related Videos

Time Multiplexing Super Resolving Technique for Imaging from a Moving Platform

Time Multiplexing Super Resolving Technique for Imaging from a Moving Platform

Published on: February 12, 2014

Simultaneous Brightfield, Fluorescence, and Optical Coherence Tomographic Imaging of Contracting Cardiac Trabeculae Ex Vivo

Simultaneous Brightfield, Fluorescence, and Optical Coherence Tomographic Imaging of Contracting Cardiac Trabeculae Ex Vivo

Published on: October 2, 2021

Related Experiment Videos

Last Updated: Apr 10, 2026

Lensfree On-chip Tomographic Microscopy Employing Multi-angle Illumination and Pixel Super-resolution

Lensfree On-chip Tomographic Microscopy Employing Multi-angle Illumination and Pixel Super-resolution

Published on: August 16, 2012

Time Multiplexing Super Resolving Technique for Imaging from a Moving Platform

Time Multiplexing Super Resolving Technique for Imaging from a Moving Platform

Published on: February 12, 2014

Simultaneous Brightfield, Fluorescence, and Optical Coherence Tomographic Imaging of Contracting Cardiac Trabeculae Ex Vivo

Simultaneous Brightfield, Fluorescence, and Optical Coherence Tomographic Imaging of Contracting Cardiac Trabeculae Ex Vivo

Published on: October 2, 2021

Area of Science:

Optics and Photonics
Biomedical Imaging
Tomography

Background:

Multi-wavelength optical diffraction tomography (ODT) provides rich contrast for biological specimens.
Existing ODT methods often require scanning or multiple exposures, limiting imaging speed and applicability.
Holographic techniques offer high-resolution imaging but integrating them with multi-wavelength ODT is challenging.

Purpose of the Study:

To develop a single-exposure system for multi-wavelength optical diffraction tomographic (ODT) imaging.
To enable rapid 3D refractive index reconstruction of specimens.
To overcome the limitations of scanning mechanisms and multiple exposures in current ODT systems.

Main Methods:

A space-angle dual multiplexing holographic recording system was designed.
The system combines angle-division multiplexing for multi-wavelength holography and microlens array multi-angle illumination for ODT.
A novel data processing method was developed for tomogram reconstruction from a single hologram.

Main Results:

The system successfully recorded all diffraction tomography information in a single camera exposure.
Multi-wavelength 3D refractive index tomograms were reconstructed from a single multiplexing hologram.
Feasibility was demonstrated using a polystyrene bead and a living C. elegans worm.

Conclusions:

The proposed space-angle dual multiplexing system enables efficient single-exposure multi-wavelength ODT imaging.
This technique significantly speeds up the acquisition of 3D refractive index information.
The system holds promise for label-free, high-resolution imaging of biological samples.