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Quality Control

Quality Control

Quality control is one of the three cyclical quality assurance activities that help keep a system under statistical control. Typical quality control activities include creating quality control charts, conducting proficiency testing, and documenting and archiving results.
Quality control helps track data, visualize trends, and identify variations, making it easier to detect deviations that may affect the accuracy of an analysis. One way to do this is by generating a quality control chart, which...

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Updated: Jul 2, 2026

Assessment of DNA Contamination in RNA Samples Based on Ribosomal DNA

Assessment of DNA Contamination in RNA Samples Based on Ribosomal DNA

Published on: January 22, 2018

Quality control in nucleic acid analysis.

¹Comprehensive Cytometric Consulting, Ballwin, Missouri, USA.

Current Protocols in Cytometry

|September 5, 2008

Summary

This summary is machine-generated.

Quality control for flow cytometry DNA analysis is crucial for accurate tumor ploidy and S-phase fraction measurements, which are vital for cancer prognosis. This ensures reliable clinical oncology data.

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NanoDrop Microvolume Quantitation of Nucleic Acids

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Area of Science:

Biomedical Engineering
Clinical Oncology
Molecular Biology

Background:

Flow cytometry is widely used for cellular analysis, including DNA content measurement.
DNA ploidy and S-phase fraction are established prognostic markers in clinical oncology.
Standard quality control (QC) for flow cytometry may not fully address DNA analysis specifics.

Purpose of the Study:

To highlight the essential quality control considerations unique to quantitative DNA analysis using flow cytometry.
To provide complementary information to existing flow cytometry QC protocols.

Main Methods:

Discussion of quality control issues specific to DNA content analysis.
Review of established flow cytometry procedures relevant to DNA analysis.

Main Results:

Identification of critical QC parameters for accurate DNA ploidy assessment.
Identification of critical QC parameters for accurate S-phase fraction determination.
Emphasis on the necessity of rigorous QC for prognostic utility.

Conclusions:

Rigorous and specific quality control is essential for reliable flow cytometry-based DNA analysis in clinical oncology.
Adherence to specialized QC procedures ensures the prognostic value of DNA ploidy and S-phase fraction measurements.
This unit complements existing protocols by focusing on DNA analysis-specific QC challenges.