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Updated: Jul 2, 2026
08:44
A High-Throughput In Situ Method for Estimation of Hepatocyte Nuclear Ploidy in Mice
Published on: April 19, 2020
DNA content measurement for DNA ploidy and cell cycle analysis
1New York Medical College, Valhalla, New York, USA.
Current Protocols in Cytometry
|September 5, 2008
Abstract:
This unit presents the most common methods of DNA content analysis. The techniques differ primarily in the mode of cell permeabilization (detergents versus prefixation with alcohol) and composition of the stain solution. Protocols are given for analysis of fixed cells, tissue samples, live cells (for subsequent sorting and culture), archival paraffin-embedded tissue, and apoptotic cells.
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