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Updated: Jul 2, 2026
07:14
A Flow Cytometry-based Assay for Measuring Mitochondrial Membrane Potential in Cardiac Myocytes After Hypoxia/Reoxygenation
Published on: July 13, 2018
Flow cytometric analysis of mitochondrial membrane potential using JC-1
1University of Modena, Modena, Italy.
Current Protocols in Cytometry
|September 5, 2008
Summary
This study highlights recent advances in fluorescent probes for measuring mitochondrial membrane potential. The JC-1 probe allows for quantitative analysis of membrane potential changes using flow cytometry.
Area of Science:
- Biochemistry
- Cell Biology
- Biophysics
Background:
- Membrane potential changes are recognized as early indicators of cellular activation.
- Accurate measurement of mitochondrial membrane potential is crucial for understanding cellular function.
Purpose of the Study:
- To present recent developments in fluorescent probes for measuring mitochondrial membrane potential.
- To demonstrate the application of the JC-1 probe for flow cytometry analysis.
- To emphasize the quantitative capabilities of this measurement technique.
Main Methods:
- Utilizing fluorescent probes, specifically the JC-1 probe.
- Employing flow cytometry for membrane potential measurements.
- Developing a system for quantitative analysis of membrane potential dynamics.
Main Results:
- Successful demonstration of the JC-1 probe for measuring mitochondrial membrane potential.
- Establishment of a flow cytometry-based method for this measurement.
- Confirmation of the system's ability to provide quantitative data on membrane potential changes.
Conclusions:
- The JC-1 probe and flow cytometry offer a robust method for assessing mitochondrial membrane potential.
- This technique enables quantitative measurement of early cellular activation events.
- Recent advancements facilitate more precise analysis of mitochondrial function.
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