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Related Concept Videos

Cell Culture01:21

Cell Culture

Most vertebrate cells grow in vitro attached to a substrate as a monolayer, called adherent cultures. The flasks and plates used to grow cells are chemically treated to facilitate cell attachment. However, a few cell types, such as hematopoietic cells, can grow in a suspension. In contrast to adherent cultures, suspension cultures can grow in non-treated cultureware using magnetic stirrers or spinner flasks to agitate the culture media

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Updated: Jul 2, 2026

Trypsinizing and Subculturing Mammalian Cells
05:59

Trypsinizing and Subculturing Mammalian Cells

Published on: June 12, 2008

Techniques for mammalian cell tissue culture.

Mary C Phelan1

  • 1Molecular Pathology Laboratory Network, Maryville, Tennessee, USA.

Current Protocols in Cytometry
|September 5, 2008
PubMed
Summary

This guide details sterile techniques and cell culture methods, including subculturing, freezing, and counting cells. It provides essential protocols for handling various cell types and preparing them for transport.

Area of Science:

  • Cell Biology
  • Microbiology
  • Biotechnology

Background:

  • Maintaining cell cultures requires strict adherence to sterile techniques.
  • Accurate cell counting and proper handling are crucial for experimental reproducibility.

Purpose of the Study:

  • To provide comprehensive protocols for essential cell culture techniques.
  • To detail the latest principles in sterile technique and media preparation.

Main Methods:

  • Sterile technique principles and culture media preparation.
  • Protocols for trypsinizing and subculturing monolayer cultures.
  • Methods for passaging suspension cultures, freezing/thawing cells, and cell counting using a hemacytometer.
  • Procedures for preparing cells for transport.

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In Vitro Cultivation Techniques for Modeling Liver Organogenesis, Building Assembloids, and Designing Synthetic Tissues using Human Cell Lines
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In Vitro Cultivation Techniques for Modeling Liver Organogenesis, Building Assembloids, and Designing Synthetic Tissues using Human Cell Lines

Published on: April 18, 2025

Scale-Up of Mammalian Cell Culture using a New Multilayered Flask
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Scale-Up of Mammalian Cell Culture using a New Multilayered Flask

Published on: December 5, 2011

Related Experiment Videos

Last Updated: Jul 2, 2026

Trypsinizing and Subculturing Mammalian Cells
05:59

Trypsinizing and Subculturing Mammalian Cells

Published on: June 12, 2008

In Vitro Cultivation Techniques for Modeling Liver Organogenesis, Building Assembloids, and Designing Synthetic Tissues using Human Cell Lines
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In Vitro Cultivation Techniques for Modeling Liver Organogenesis, Building Assembloids, and Designing Synthetic Tissues using Human Cell Lines

Published on: April 18, 2025

Scale-Up of Mammalian Cell Culture using a New Multilayered Flask
08:39

Scale-Up of Mammalian Cell Culture using a New Multilayered Flask

Published on: December 5, 2011

Main Results:

  • Detailed step-by-step protocols for various cell culture manipulations.
  • Emphasis on the latest advancements in sterile procedures.
  • Clear guidelines for cell enumeration and preservation.

Conclusions:

  • Mastery of these techniques is fundamental for successful cell-based research.
  • The unit equips researchers with practical skills for routine cell culture laboratory tasks.