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Updated: Jun 7, 2026

05:59
Trypsinizing and Subculturing Mammalian Cells
Published on: June 12, 2008
Techniques for mammalian cell tissue culture
1Molecular Pathology Laboratory Network, Maryville, Tennessee, USA.
Current Protocols in Toxicology
|October 26, 2010
Abstract:
This appendix opens with detailed discussions on the latest principles of sterile technique and preparation of culture media. Step-by-step protocols describe trypsinizing and subculturing monolayer cultures, passaging suspension cultures, freezing and thawing cells, counting cells using a hemacytometer, and preparing cells for transport.
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