Related Experiment Video
Updated: Jul 2, 2026
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Trypsinizing and Subculturing Mammalian Cells
Published on: June 12, 2008
Techniques for mammalian cell tissue culture
1Molecular Pathology Laboratory Network, Maryville, Tennessee, USA.
Current Protocols in Cytometry
|September 5, 2008
Summary
This guide details sterile techniques and cell culture methods, including subculturing, freezing, and counting cells. It provides essential protocols for handling various cell types and preparing them for transport.
Area of Science:
- Cell Biology
- Microbiology
- Biotechnology
Background:
- Maintaining cell cultures requires strict adherence to sterile techniques.
- Accurate cell counting and proper handling are crucial for experimental reproducibility.
Purpose of the Study:
- To provide comprehensive protocols for essential cell culture techniques.
- To detail the latest principles in sterile technique and media preparation.
Main Methods:
- Sterile technique principles and culture media preparation.
- Protocols for trypsinizing and subculturing monolayer cultures.
- Methods for passaging suspension cultures, freezing/thawing cells, and cell counting using a hemacytometer.
- Procedures for preparing cells for transport.
Main Results:
- Detailed step-by-step protocols for various cell culture manipulations.
- Emphasis on the latest advancements in sterile procedures.
- Clear guidelines for cell enumeration and preservation.
Conclusions:
- Mastery of these techniques is fundamental for successful cell-based research.
- The unit equips researchers with practical skills for routine cell culture laboratory tasks.
