Related Experiment Video
Updated: Jul 5, 2026

05:59
Trypsinizing and Subculturing Mammalian Cells
Published on: June 12, 2008
Techniques for mammalian cell tissue culture
1Molecular Pathology Laboratory Network, Maryville, Tennessee, USA.
Current Protocols in Neuroscience
|April 23, 2008
Abstract:
This unit opens with detailed discussions on the latest principles of sterile technique and preparation of culture media. Step-by-step protocols describe trypsinizing and subculturing monolayer cultures, passaging suspension cultures, freezing and thawing cells, counting cells using a hemacytometer, and preparing cells for transport.

