Standard safety practices for sorting of unfixed cells

Area of Science:

• Cell Biology
• Biotechnology
• Laboratory Safety

Background:

• Viable cell sorting is a common technique in basic and clinical research.
• Handling biological specimens poses potential infectious risks to operators and the environment.
• Existing biosafety guidelines require updating to reflect current practices.

Purpose of the Study:

• To present revised and updated biosafety guidelines for viable cell sorting.
• To provide comprehensive recommendations for safe laboratory practices.
• To ensure protection of personnel and the environment from biohazards.

Main Methods:

• Review and update established biosafety guidelines from the International Society of Analytical Cytology (ISAC).
• Incorporate background information on biohazard potential and infectious agent classification.
• Develop recommendations covering sample handling, operator training, personal protection, laboratory design, instrument setup, maintenance, decontamination, and aerosol containment testing.

Main Results:

• The unit offers a comprehensive framework for biosafety in cell sorting.
• Specific recommendations are provided for all critical aspects of the cell sorting workflow.
• The updated guidelines aim to establish worldwide standard practices.

Conclusions:

• Adherence to these updated biosafety guidelines is crucial for safe cell sorting.
• Implementing these recommendations minimizes risks associated with infectious agents in cell sorting.
• These guidelines support the responsible advancement of cell sorting applications in research.