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Related Experiment Video

Updated: Jul 2, 2026

A Protocol for Real-time 3D Single Particle Tracking
10:16

A Protocol for Real-time 3D Single Particle Tracking

Published on: January 3, 2018

Fast, bias-free algorithm for tracking single particles with variable size and shape.

Andrew J Berglund1, Matthew D McMahon, Jabez J McClelland

  • 1Center for Nanoscale Science and Technology, National Institute of Standards and Technology, Gaithersburg, MD 20899, USA. andrew.berglund@nist.gov

Optics Express
|September 6, 2008
PubMed
Summary
This summary is machine-generated.

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We developed a fast and accurate single-particle tracking method. This technique precisely locates particles without needing prior image information, enabling real-time applications.

Area of Science:

  • Biophysics
  • Optical Microscopy
  • Nanotechnology

Background:

  • Accurate single-particle tracking is crucial for understanding biological processes at the nanoscale.
  • Existing methods often require prior knowledge of particle characteristics or complex computations.
  • Background noise and simplistic centroid estimation can introduce significant bias.

Purpose of the Study:

  • To introduce a novel, fast, and robust algorithm for single-particle tracking.
  • To achieve nanometer-level localization accuracy.
  • To develop a method suitable for hardware implementation and real-time feedback.

Main Methods:

  • A simple, iterative algorithm to extract the center-of-mass of particle images.
  • Suppression of background-induced bias in centroid estimation.

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Controlled Synthesis and Fluorescence Tracking of Highly Uniform Poly(N-isopropylacrylamide) Microgels
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Controlled Synthesis and Fluorescence Tracking of Highly Uniform Poly(N-isopropylacrylamide) Microgels

Published on: September 8, 2016

Image Processing Protocol for the Analysis of the Diffusion and Cluster Size of Membrane Receptors by Fluorescence Microscopy
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Image Processing Protocol for the Analysis of the Diffusion and Cluster Size of Membrane Receptors by Fluorescence Microscopy

Published on: April 9, 2019

Related Experiment Videos

Last Updated: Jul 2, 2026

A Protocol for Real-time 3D Single Particle Tracking
10:16

A Protocol for Real-time 3D Single Particle Tracking

Published on: January 3, 2018

Controlled Synthesis and Fluorescence Tracking of Highly Uniform Poly(N-isopropylacrylamide) Microgels
11:34

Controlled Synthesis and Fluorescence Tracking of Highly Uniform Poly(N-isopropylacrylamide) Microgels

Published on: September 8, 2016

Image Processing Protocol for the Analysis of the Diffusion and Cluster Size of Membrane Receptors by Fluorescence Microscopy
12:15

Image Processing Protocol for the Analysis of the Diffusion and Cluster Size of Membrane Receptors by Fluorescence Microscopy

Published on: April 9, 2019

  • Demonstration using numerical simulations and experimental data with fluorescent microspheres.

    • Main Results:

    • The algorithm achieves nanometer accuracy in single-particle localization.
    • It requires no prior information on particle shape or size.
    • Localization of 190 nm fluorescent microspheres to better than 5 nm was achieved using an inexpensive CCD camera.

    Conclusions:

    • The developed technique offers a fast, robust, and accurate solution for single-particle tracking.
    • Its simplicity and minimal requirements make it ideal for real-time applications and hardware integration.
    • This method advances capabilities in nanoscale imaging and analysis.