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Updated: Jul 1, 2026

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In Vitro Directed Evolution of a Restriction Endonuclease with More Stringent Specificity
Published on: March 25, 2020
In vitro selection of a DNAzyme with three modified nucleotides
Marcel Hollenstein1, Christopher Hipolito, Curtis Lam
1Department of Chemistry, University of British Columbia, 2036 Main Mall, Vancouver BC, V6T 1Z1, Canada.
Nucleic Acids Symposium Series (2004)
|September 9, 2008
Abstract:
Three modified nucleosides have been used in the in vitro selection of the self-cleaving DNAzyme 9-33. This DNAzyme operates in the absence of divalent metal cations with a first-order constant of approximately 0.05 min(-1).

