Related Experiment Video
Updated: Jul 1, 2026

06:49
Lentiviral Mediated Delivery of shRNAs to hESCs and NPCs Using Low-cost Cationic Polymer Polyethylenimine (PEI)
Published on: May 24, 2022
Tyrosine-modified PEI: a novel and highly efficient vector for siRNA delivery in mammalian cells
1Laboratoire de Chimie Génétique, CNRS-ULP UMR 7175, Faculté de Pharmacie, 74, route du Rhin, 67400 Illkirch, France.
Nucleic Acids Symposium Series (2004)
|September 9, 2008
Abstract:
Delivery of synthetic small interfering RNA (siRNA) into cell remains the major obstacle to its biological activity. Cationic polymers, and in particular the "proton sponge" polyethylenimine (PEI), has shown promise for cancer gene therapy but appears inefficient for siRNA delivery. Here we report that modifying branched PEI with amino acids led to efficient siRNA delivery into mammalian cell lines, even in the presence of serum, and at dose as low as 1 nM.
