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Updated: Jul 1, 2026

In Vitro Chemical Mapping of G-Quadruplex DNA Structures by Bis-3-Chloropiperidines
Published on: May 12, 2023
Orientation of ends of G-quadruplex structure investigated with end-extended oligonucleotides
Yuta Sannohe1, Kyosuke Sato, Akimasa Matsugami
1Department of Chemistry, Graduate School of Science, Kyoto University, Kitashirakawa-Oiwakecho, Sakyo, Kyoto 606-8502, Japan.
Abstract:
The human telomere terminus can adopt the structure of a G-quadruplex. This structure has become an attractive target for anticancer drugs, because it effectively inhibits telomerase activity. In this study, we investigated the orientation of both 5' and 3' ends of the stable G-quadruplex structure. To verify the orientation, we designed end-extended G-quadruplex forming oligonucleotides. We carried out gel electrophoresis and the NMR analysis and found that the ends of the stable G-quadruplex structure are located on opposite faces of each of the quadruplexes.
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