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Updated: Jul 1, 2026
12:29
Investigation of Protein Recruitment to DNA Lesions Using 405 Nm Laser Micro-irradiation
Published on: March 20, 2018
SPR-imaging based assays on an oligonucleotide-array to analyze DNA lesions recognition and excision by repair
1Laboratoire Lésions des Acides Nucléiques, SCIB/UMR E3 CEA-UJF, INAC, CEA-Grenoble, F-38054 Grenoble Cedex 9, France.
Nucleic Acids Symposium Series (2004)
|September 9, 2008
Abstract:
An original oligonucleotide-array, coupled with SPR-imaging detection, has been developed to study biological interactions between DNA base lesions and DNA repair enzymes. This bioanalytical tool constitutes an efficient screening platform to quantify DNA repair activities and to search for new DNA repair inhibitors.
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Cells are regularly exposed to mutagens—factors in the environment that can damage DNA and generate mutations. UV radiation is one of the most common mutagens and is estimated to introduce a significant number of changes in DNA. These include bends or kinks in the structure, which can block DNA replication or transcription. If these errors are not fixed, the damage can cause mutations, which in turn can result in cancer or disease depending on which sequences are...
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The first step of...
The first step of...