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Updated: Jul 1, 2026
Chemical Triphosphorylation of Oligonucleotides
Published on: June 2, 2022
Optimized fluoride ions treatment for release of base-sensitive 2'-O-modified oligoribonucleotides from various solid
Thomas Lavergne1, Françoise Debart, Jean-Jacques Vasseur
1IBMM, UMR 5247 CNRS-UM1-UM2, CC 1704, Université Montpellier 2, Place Eugène Bataillon, 34095 Montpellier Cedex 5, France.
Abstract:
To synthesize oligoribonucleotides containing base-sensitive 2'-O-modifications, we used the Q-linker or a silyl linker anchored to CPG or polystyrene resin. A fluoride ions treatment was optimized to release oligoribonucleotides from the solid-support and to remove TBDMS groups without affecting 2'-O-acyloxymethyl groups.
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