Fluorogenic probe triggered by reduction for nucleic acids sensing.

Area of Science:

• Analytical Chemistry
• Biochemistry
• Molecular Biology

Background:

• Oligonucleotide detection is crucial for molecular diagnostics and biological research.
• Existing methods for sensing nucleic acids can be limited by sensitivity, speed, or the need for specific conditions.
• Development of rapid, sensitive, and biologically compatible probes is an ongoing challenge.

Purpose of the Study:

• To develop a novel reduction-triggered fluorescence probe for sensitive oligonucleotide detection.
• To utilize a rhodamine 110 derivative activated by azide reduction for signal generation.
• To demonstrate the probe's efficacy in biological settings, including bacterial cells.

Main Methods:

• Synthesis of a rhodamine 110 derivative featuring an azide group.
• Investigating the reduction of the azide group by reducing reagents to trigger fluorescence.
• Assessing the probe's reaction kinetics and fluorescence response in the presence of target DNA and RNA.
• Applying the probe for detecting oligonucleotides in solution and endogenous RNA within bacterial cells.

Main Results:

• A new fluorogenic compound derived from rhodamine 110 was successfully synthesized.
• The probe exhibited fluorescence signal generation upon reduction of the azide group.
• The reaction proceeded efficiently under biological conditions, yielding results within 10-20 minutes.
• Successful detection of oligonucleotides in solution and endogenous RNA in bacterial cells was achieved.

Conclusions:

• A novel reduction-triggered fluorescence probe for oligonucleotide sensing has been developed.
• The probe demonstrates rapid and sensitive detection capabilities under physiological conditions.
• This technology holds promise for applications in molecular diagnostics and biological imaging.