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Updated: Jul 1, 2026
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Photoactivated Localization Microscopy with Bimolecular Fluorescence Complementation (BiFC-PALM)
Published on: December 22, 2015
Site-specific modification by functionality-transfer oligonucleotide with the photo-inducible reactivity
Kazumitsu Onizuka1, Yosuke Taniguchi, Takamasa Nishioka
1Graduate School of Pharmaceutical Sciences, Kyushu University, Fukuoka 812-8582, Japan.
Nucleic Acids Symposium Series (2004)
|September 9, 2008
Summary
Researchers developed photo-inducible functionality-transfer oligonucleotides. These novel molecules selectively react with modified cytidine (d(m)C) in DNA, enabling targeted modifications upon light activation.
Area of Science:
- Oligonucleotide chemistry
- Photochemistry
- Molecular biology
Background:
- S-vinyl thioguanosine analogs enable selective functionality transfer to cytidine in nucleic acids.
- This methodology has been applied for DNA/RNA inhibition and labeling.
Purpose of the Study:
- To develop photo-inducible functionality-transfer oligonucleotides.
- To achieve light-triggered, site-specific modifications in DNA.
Main Methods:
- Synthesis of S-vinyl thioguanosine analogs with photo-inducible reactivity.
- Application of these analogs in oligonucleotide synthesis.
- Testing the reactivity and selectivity of the photo-induced transfer reaction on complementary DNA targets.
Main Results:
- The developed functionality-transfer oligonucleotides exhibit photo-inducible reactivity.
- The photo-induced transfer reaction shows high selectivity, occurring exclusively at the modified cytidine (d(m)C) site.
- No reaction was observed with unmodified cytidine (dC) or thymidine (dT) at the target site.
Conclusions:
- Photo-inducible functionality-transfer oligonucleotides offer a novel approach for site-specific DNA modification.
- This technique allows for precise control over modification through light activation.
- The selectivity for d(m)C opens avenues for targeted nucleic acid manipulation and labeling.
