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New methodology to search for DNA binding ligands based on duplex DNA-templated click chemistry

Shuhei Imoto1, Tomoya Hirohama, Fumi Nagatsugi

  • 1Institute of Multidisciplinary Research for Advanced Materials, Tohoku University, Sendai-city, Miyagi, Japan.

Nucleic Acids Symposium Series (2004)
|September 9, 2008
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Labeling DNA Probes03:31

Labeling DNA Probes

DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...