Radiolytic ligation of oligodeoxynucleotides possessing disulfide bond
Kazuhito Tanabe1, Emi Kuraseko, Eiji Matsumoto
1Department of Energy and Hydrocarbon Chemistry, Graduate School of Engineering, Kyoto University, Katsura Campus, Kyoto 615-8510, Japan. tanabeka@scl.kyoto-u.ac.jp
Nucleic Acids Symposium Series (2004)
|September 9, 2008
Abstract:
Hypoxic X-radiolysis of diluted aqueous solutions was performed to generate hydrated electrons that induced one-electron reduction of oligodeoxynucleotides (ODNs) possessing a disulfide bond. Upon hypoxic irradiation, reductive ligation reaction between two types of ODNs occurred in the presence of a template ODN strand, resulting in the formation of a prescribed ODN in substantial amounts.
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