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Updated: Jul 1, 2026
An Optimized Quantitative Pull-Down Analysis of RNA-Binding Proteins Using Short Biotinylated RNA
Published on: February 17, 2023
In vitro selection of RNA aptamer to hemin
Mingzhe Liu1, Takuma Kagahara, Hiroshi Abe
1Nano Medical Engineering Laboratory, RIKEN-The Institute of Physical and Chemical Research, Wako-shi, Saitama, 351-0198, Japan.
Abstract:
A new RNA aptamer-binding hemin was synthesized by the in vitro selection (SELEX) method. A pool of 103 bases single strand DNAs containing a randomized sequence of 59 bases was synthesized. The pool was incubated with hemin on hemin-immobilized affinity column. Bound RNAs were eluted off with hemin solution and amplified by PCR. After 3 rounds of selection process, the selected RNAs were cloned and sequenced. Some RNA aptamers, which have affinity to hemin, was selected.
