Related Experiment Video
Updated: Jul 1, 2026
08:23
Design and Development of Aptamer–Gold Nanoparticle Based Colorimetric Assays for In-the-field Applications
Published on: June 23, 2016
Simple and rapid colorimetric detection of low-weight molecules using aptazymes in combination with noncrosslinking
1Bioengineering Laboratory, RIKEN-The Institute of Physical and Chemical Research, Wako, Saitama 351-0198, Japan. a-ogawa@riken.jp
Nucleic Acids Symposium Series (2004)
|September 9, 2008
Abstract:
We developed a method for simply and rapidly detecting cofactors of aptazymes with high sensitivity using a unique noncrosslinking gold nanoparticle aggregation. Applying this method to a theophylline dependent aptazyme, 100 microM, 10 microM, and 1 microM theophylline were detected easily by the naked eye within 10 min, 20 min, and 65 min, respectively. This method is applicable to other cleavage-aptazymes without altering the probe-DNA sequence.
