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Updated: Jul 1, 2026

Evaluation of Polymeric Gene Delivery Nanoparticles by Nanoparticle Tracking Analysis and High-throughput Flow Cytometry
Published on: March 1, 2013
Improvement of transcription- and transfection-efficiency by synthetic polyampholytes
Chieko Yoshihara1, Tomoko Ito, Chwen-Yang Shew
1Otsuma Women's University Graduate School, Chiyoda-ku, Tokyo, Japan. d1107101@cst.otsuma.ac.jp
Abstract:
Water-soluble PEG derivatives having both amino- and carboxyl-pendants (PEG-ACs) were synthesized, and examined for their transcription- and transfection-enhancing activity on DNA/polycation complexes. PEG-AC could be deposited onto the surface of DNA/polyethylenimine(PEI) complexes, and enhanced their transcriptional activity. Fluorescence anisotropy study showed that the amphoteric PEG-AC loosened the tightly compacted DNA/PEI complex to facilitate the approach of transcriptional factors. Transcriptional activity of the complex was in good correlation with its transgene expression efficiency, indicating the importance of improvement in transcription for effective gene transfection.

