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Updated: Jul 1, 2026
Preparation of Expanded Chitin Foams and their Use in the Removal of Aqueous Copper
Published on: February 27, 2021
Environment friendly crosslinked chitosan as a matrix for selective adsorption and purification of lipase of
K D Trimukhe1, N D Mahadik, D V Gokhale
1Polymer Science and Engineering Division, National Chemical Laboratory, Pune 411008, India.
Abstract:
Chitosan and its derivatives have been used as affinity matrices for purification of lipase from Aspergillus niger NCIM 1207. Trimellitic anhydride (TMA)-crosslinked deacetylated chitin adsorbed lipase selectively, yielding approximately 5-fold purification of the crude lipase with 70% yield. Further 9-fold purification occurred on eluting through Sephacryl-100. These results suggest that chitosan derivatives can be used as inexpensive biopolymer matrices for the purification of lipases for industrial applications.
