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Updated: Jun 30, 2026
Detection and Genogrouping of Noroviruses from Children's Stools By Taqman One-step RT-PCR
Published on: July 22, 2012
[Rotaviruses in younger children in Novosibirsk in 2005-2007: detection and genotyping]
Insights
Group A rotaviruses frequently cause acute gastroenteritis in children under three, particularly infants. Mixed infections with other pathogens are common, with P[8]G1 being the most prevalent rotavirus strain.
Area of Science:
- Virology
- Pediatric Infectious Diseases
- Microbiology
Context:
- Acute gastroenteritis is a significant health concern in young children.
- Rotavirus is a leading cause of severe diarrheal disease globally.
- Understanding local epidemiology and circulating strains is crucial for public health interventions.
Purpose:
- To identify the primary etiological agents of acute enteric infections in children under three years.
- To characterize the prevalent group A rotavirus strains and their genotypes.
- To investigate the occurrence and patterns of mixed infections.
Summary:
- A study of 1898 patients revealed group A rotaviruses as the main cause (35%) of acute gastroenteritis in children under three, with most cases in infants under one year.
- Mixed infections (45.6%) involving rotaviruses and other viruses or bacteria were frequently detected.
- Genotyping identified P[8]G1 as the most common group A rotavirus strain (54.6%), followed by P[8]G3, P[8]G9, P[4]G2, and P[8]G4.
Impact:
- Provides essential epidemiological data on rotavirus prevalence and strain types in a specific pediatric population.
- Highlights the significance of rotavirus in pediatric gastroenteritis and the complexity of mixed infections.
- Informs vaccine strategies and clinical management of acute enteric infections in children.
Abstract:
Examination of 1898 patients with acute enteric infection from March 2005 to February 2007 showed that group A rotaviruses were the most frequent cause (35%) of acute gastroenteritis among children under 3 years of age. Majority of cases of rotavirus infection was detected in infants under 1 year of age (71.8%). The peak of sporadic incidence was observed between February and May. High rate of mixed infection (45.6%) was observed - associations of rotaviruses with other viruses (noroviruses, astroviruses) and bacteria (Salmonella, Shigella, enteroinvasive Escherichia coli, Campylobacter, and opportunistic species) were detected. P- and G-genotypes of 337(50.8%) isolates of group A rotaviruses were determined by RT-PCR. The most prevalent strain was P[8]G1 (54.6%) followed by P[8]G3 (10.7%), P[8]G9 (8.6%), P[4]G2 (8.3%), and P[8]G4 (4.5%) genotypes.
