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Updated: Jun 30, 2026

Total Protein Extraction and 2-D Gel Electrophoresis Methods for Burkholderia Species
Published on: October 15, 2013
[Phenotypic and genotypic identification of bacteria from the Burkholderia cepacia complex]
Aim:
Evaluation of the diagnostic value of pheno- and genotypic characteristics of B. cepacia strains collection.
Materials And Methods:
Phenotypic and genetic methods of identification and differentiation of 25 strains of the B. cepacia complex.
Results:
Polyphasic taxonomic approach utilizing multiple diagnostic tests was used for accurate identification of Burkholderia species. Algorithm for identification of microorganisms from the B. cepacia complex was developed.
Conclusion:
Combined use of phenotypic and molecular genetic tests, such as recA gene PCR, is recommended for differentiation of the B. cepacia complex genomovars.
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