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Affinity processing of cell-containing feeds using monolithic macroporous hydrogels, cryogels.

Igor Y Galaev1, Bo Mattiasson

  • 1Department of Biotechnology, Centre for Chemistry and Chemical Engineering, Lund University, Lund, Sweden.

Methods in Molecular Biology (Clifton, N.J.)
|October 2, 2008
PubMed
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Monolithic macroporous hydrogels, called cryogels, offer unique properties like large pores and minimal interactions. These characteristics enable efficient protein capture and cell separation applications.

Area of Science:

  • Biomaterials Science
  • Chemical Engineering
  • Biotechnology

Background:

  • Macroporous hydrogels, known as cryogels, are synthesized via polymerization in a partially frozen state.
  • Ice crystals act as porogens during cryogel formation, creating a unique porous structure.

Purpose of the Study:

  • To highlight the distinct properties of cryogels.
  • To explore the applications of cryogels in bioseparation processes.

Main Methods:

  • Synthesis of monolithic macroporous hydrogels (cryogels) through polymerization in a partially frozen state.
  • Characterization of cryogel properties including pore size, porosity, and mechanical stability.

Main Results:

  • Cryogels exhibit large pore sizes (10-100 micrometers).

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  • High porosities (exceeding 80-90%) and minimal non-specific interactions due to hydrophilic polymer nature.
  • Good mechanical stability was observed in the synthesized cryogels.

    • Conclusions:

    • Cryogels possess a unique combination of properties suitable for bioseparation.
    • The properties of cryogels facilitate direct capture of extracellularly expressed histidine-tagged protein from fermentation broth.
    • Cryogels show potential for effective separation of different cell types.