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Updated: Oct 7, 2025

Ultrasensitive Detection of Biomarkers by Using a Molecular Imprinting Based Capacitive Biosensor

Ultrasensitive Detection of Biomarkers by Using a Molecular Imprinting Based Capacitive Biosensor

Published on: February 16, 2018

A Sensitive Capacitive Biosensor for Protein a Detection Using Human IgG Immobilized on an Electrode Using

Kosin Teeparuksapun^1,2, Martin Hedström², Bo Mattiasson²

¹Science Program, Department of General Education, Faculty of Liberal Arts, Rajamangala University of Technology Srivijaya, Songkhla 90000, Thailand.

Sensors (Basel, Switzerland)

|January 11, 2022

Summary

A new capacitive biosensor detects protein A using gold electrodes and layer-by-layer assembly. Optimized probe height enhances sensitivity for detecting trace amounts of protein A in antibody isolation.

Keywords:

capacitive biosensor gold nanoparticle layer-by-layer protein A thiourea

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Last Updated: Oct 7, 2025

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Published on: November 10, 2016

Area of Science:

Biosensing
Nanotechnology
Electrochemistry

Background:

Protein A is crucial in purifying therapeutic monoclonal antibodies.
Capacitive biosensors offer label-free detection methods.
Optimizing probe architecture is key for biosensor sensitivity.

Purpose of the Study:

To develop a capacitive biosensor for sensitive protein A detection.
To investigate the impact of layer-by-layer (LbL) probe height on assay sensitivity.
To establish a quantitative detection range and limit of detection for protein A.

Main Methods:

Fabrication of gold electrodes via thermal evaporation and photolithography.
Layer-by-layer assembly of thiourea and gold nanoparticles (AuNPs) to create probes.
Immobilization of human IgG and detection of protein A binding via capacitance changes.

Main Results:

Biosensor sensitivity increased with initial probe layer growth.
Excessive probe thickness led to decreased measurement sensitivity.
Linear detection range from 1 x 10^-16 to 1 x 10^-13 M was achieved.
Limit of detection was determined to be 9.1 x 10^-17 M.

Conclusions:

The developed capacitive biosensor effectively detects protein A.
Probe height optimization is critical for maximizing biosensor performance.
The system shows potential for detecting trace protein A in antibody purification processes.