Genetic changes induced by the pesticide Ridomol in the soybeans sprouted from the seeds of different ages

An objective of presented study was to investigate the effect of the genetically active xenobiotics Ridomili on the genetic apparatus of the plants sprouted from the soybean seeds of different ages. The experiments were conducted on the soybean (Glycine max) genetic line L65-1237. The studies were performed on the 1-, 2- or 3- year old seeds of the heterozygous Y(11)y(11) plants. The seeds of different ages were exposed to the pesticide Ridomili diluted in distilled water in different concentrations--0.02%, 0.04%, 0.06%, 0.08% and 0.1%. Three phenotype classes--green (Y(11)Y(11)), light-green (Y(11)y(11)) and yellow (y(11)y(11)) in proportion 1:2:1. All the concentrations of the substance caused mitotic crossingovers in the plants sprouted from the 3-year-old seeds. The higher doses of Ridomil (0.06-0.1%) caused induction of reversions much more frequently than in the case of 1- and 2-year-old seeds. The tendency to the dose-dependant effect was also observed. Embryonic cells of 3-year-old seeds were found to be highly sensitive. The levels of direct mutations were rather common there.