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Detection of Toxin Translocation into the Host Cytosol by Surface Plasmon Resonance
Published on: January 3, 2012
Surface plasmon resonance assay for chloramphenicol.
Jing Yuan1, Richard Oliver, Marie-Isabel Aguilar
1Biosensors and Biomeasurement, The Horticulture and Food Research Institute of New Zealand, HortResearch Ruakura, Private Bag 3123, Waikato Mail Centre, Hamilton, New Zealand.
Analytical Chemistry
|October 8, 2008
Summary
This study presents a fast and sensitive surface plasmon resonance (SPR) assay for detecting chloramphenicol (CAP). The enhanced method uses gold nanoparticles for improved signal detection, achieving ultra-low limits for this important antibiotic.
Area of Science:
- Analytical Chemistry
- Biosensing Technology
- Nanotechnology
Background:
- Chloramphenicol (CAP) is a widely used antibiotic with potential health risks at high concentrations.
- Accurate and sensitive detection methods are crucial for monitoring CAP residues in food and environmental samples.
- Existing detection methods may lack the speed, sensitivity, or stability required for routine analysis.
Purpose of the Study:
- To develop a rapid and ultrasensitive surface plasmon resonance (SPR) assay for chloramphenicol (CAP) detection.
- To enhance the assay's sensitivity using large gold nanoparticles (40 nm) and a mixed self-assembled monolayer (mSAM) sensor surface.
- To evaluate the assay's performance in aqueous buffer and a complex matrix like honey.
Main Methods:
- Immobilization of CAP-ovalbumin (OVA) conjugates on an mSAM surface via an oligoethylene glycol (OEG) linker.
- Sequential binding of anti-CAP antibody and IgG/nanogold (40 nm) for signal amplification.
- Utilizing surface plasmon resonance (SPR) spectroscopy for real-time detection and quantification.
Main Results:
- Achieved an ultra-low limit of detection (LOD) of 0.74 fg/mL for CAP in aqueous buffer.
- Established a linear working range of 1-1000 fg/mL in buffer.
- Demonstrated a sensitive LOD of 17.5 fg/mL in honey, with a detection range of 80-5000 fg/mL.
- Confirmed high sensor stability with over 400 binding/regeneration cycles.
Conclusions:
- The developed SPR assay offers a rapid (<10 min) and highly sensitive method for CAP detection.
- The use of large gold nanoparticles and mSAM significantly enhances assay performance.
- The assay shows promise for reliable and stable monitoring of CAP in various sample types.
