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[Effects of toll-like-receptor-9 expression in chronic rhinosinusitis with nasal polyps]
Zhongfang Xia1, Weijia Kong, Jianxin Yue
1Department of Otolaryngology, Union Hospital Affiliated of Huazhong University of Science and Technology, Wuhan, 430022, China.
Objective:
To determine the presence of TLR-9 protein in primary epithelial cell cultures of chronic rhinosinusitis with nasal polyps (CRSwNP), and then explore the role of innate immune recognition in the pathogenesis of CRSwNP.
Method:
Primary epithelial cell cultures were established in 10 controls and 10 CRSwNP patients who underwent sinus surgery, and flow cytometry was used to confirm its purity and measure the expression of TLR-9 protein.
Result:
By digestive method 98% of cultured primary nasal mucosa cells were epithelial cells and the expression of TLR-9 protein in CRSwNP group (11%-15%) was lower than that in normal group (49%-60%).
Conclusion:
This finding suggests that impaired innate immune responses via TLR-9 on sinonasal epithelial cells may involve in pathogenesis of CRSwNP.
