Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Diagnosis of infections in newborns using a new particle-mediated immunoassay for serum C-reactive protein.

The Journal of automatic chemistry·2008
Same author

First basic performance evaluation of the XE-2100 haematology analyser.

Journal of automated methods & management in chemistry·2008
Same author

Evaluation of use of the optional unit QA-810V for the determination of five-part leukocyte differentials.

Journal of automated methods & management in chemistry·2008
Same author

CTAD as a universal anticoagulant.

Journal of automated methods & management in chemistry·2008
Same author

Toward an interpretation of dynamic neural activity in terms of chaotic dynamical systems.

The Behavioral and brain sciences·2002
Same author

Flow cytometric analysis of platelet activation under calcium ion-chelating conditions.

Clinical and laboratory haematology·2002

Related Experiment Video

Updated: Jun 28, 2026

Comprehensive Protocol to Sample and Process Bone Marrow for Measuring Measurable Residual Disease and Leukemic Stem Cells in Acute Myeloid Leukemia
09:57

Comprehensive Protocol to Sample and Process Bone Marrow for Measuring Measurable Residual Disease and Leukemic Stem Cells in Acute Myeloid Leukemia

Published on: March 5, 2018

Automated bone marrow analysis using the CD4000 automated haematology analyser.

R Yamamura1, T Yamane, M Hino

  • 1Department of Clinical Hematology, Osaka City University Medical School 1-4-3 Asahimachi, Abeno-ku, Osaka, Japan.

Journal of Automated Methods & Management in Chemistry
|October 18, 2008
PubMed
Summary
This summary is machine-generated.

Automated bone marrow analysis using the CELL-DYN 4000 (CD4000) hematology analyzer shows promise. The resistant red blood cell (RBC) mode provides results closer to traditional microscopic analysis, improving erythroblast and M/E ratio accuracy.

More Related Videos

Database-guided Flow-cytometry for Evaluation of Bone Marrow Myeloid Cell Maturation
12:05

Database-guided Flow-cytometry for Evaluation of Bone Marrow Myeloid Cell Maturation

Published on: November 3, 2018

Comprehensive & Cost Effective Laboratory Monitoring of HIV/AIDS: an African Role Model
23:56

Comprehensive & Cost Effective Laboratory Monitoring of HIV/AIDS: an African Role Model

Published on: October 31, 2010

Related Experiment Videos

Last Updated: Jun 28, 2026

Comprehensive Protocol to Sample and Process Bone Marrow for Measuring Measurable Residual Disease and Leukemic Stem Cells in Acute Myeloid Leukemia
09:57

Comprehensive Protocol to Sample and Process Bone Marrow for Measuring Measurable Residual Disease and Leukemic Stem Cells in Acute Myeloid Leukemia

Published on: March 5, 2018

Database-guided Flow-cytometry for Evaluation of Bone Marrow Myeloid Cell Maturation
12:05

Database-guided Flow-cytometry for Evaluation of Bone Marrow Myeloid Cell Maturation

Published on: November 3, 2018

Comprehensive & Cost Effective Laboratory Monitoring of HIV/AIDS: an African Role Model
23:56

Comprehensive & Cost Effective Laboratory Monitoring of HIV/AIDS: an African Role Model

Published on: October 31, 2010

Area of Science:

  • Hematology
  • Clinical Pathology
  • Cellular Analysis

Background:

  • Microscopic bone marrow analysis is labor-intensive and time-consuming.
  • Existing automated analyzers struggle with erythroblast identification, limiting automation.
  • Automated methods are needed to improve efficiency and accuracy in bone marrow cell classification.

Purpose of the Study:

  • To evaluate the automated analysis of bone marrow aspirates using the CELL-DYN 4000 (CD4000) hematology analyzer.
  • To compare automated erythroblast counts with microscopic differential counts.
  • To assess the utility of normal and resistant red blood cell (RBC) modes for bone marrow analysis.

Main Methods:

  • Automated analysis of 98 bone marrow aspirates using the CD4000 in normal and resistant RBC modes.
  • Microscopic differential counts of May-Grünwald-Giesa-stained bone marrow films as a reference method.
  • Statistical correlation analysis between automated and microscopic results for cell populations and M/E ratio.

Main Results:

  • Significant correlations (P < 0.01) were found between CD4000 and microscopic methods for total nucleated cells, lymphocytes, neutrophils, erythroblasts, and M/E ratio.
  • The CD4000 normal mode showed biases towards lower percentages for erythroblasts, lymphocytes, and M/E ratio compared to microscopy.
  • The CD4000 resistant RBC mode yielded results that closely approximated microscopic findings for these parameters.

Conclusions:

  • The CD4000 hematology analyzer can automate bone marrow analysis, including erythroblast counts.
  • The resistant RBC mode of the CD4000 is superior to the normal mode for bone marrow analysis.
  • The resistant RBC mode provides a more accurate approximation of the M/E ratio, making it more useful for clinical applications.