Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Anticoagulant Drugs: Vitamin K Antagonists and Direct Oral Anticoagulants

Anticoagulant Drugs: Vitamin K Antagonists and Direct Oral Anticoagulants

Oral anticoagulants are vital tools in preventing and treating blood clotting disorders. This diverse class of medications can be categorized as vitamin K antagonists, exemplified by warfarin, and direct thrombin inhibitors (DTIs), such as dabigatran, as well as factor Xa inhibitors, including rivaroxaban.
Warfarin, a prominent vitamin K antagonist family member, exerts its effect by inhibiting the enzyme VKORC1 (vitamin K epoxide reductase complex 1). By hindering this enzyme, warfarin...

Anticoagulant Drugs: Low-Molecular-Weight Heparins

Anticoagulant Drugs: Low-Molecular-Weight Heparins

Hemostasis is a crucial process that prevents excessive blood loss from damaged blood vessels. It involves various mechanisms such as vasoconstriction, platelet adhesion and activation, and fibrin formation. The importance of each mechanism depends on the type of vessel injury. In contrast, thrombosis is the abnormal formation of a blood clot within the blood vessels, leading to potential complications if the clot obstructs blood flow. Thrombosis can be caused by increased coagulability of the...

EDTA: Chemistry and Properties

EDTA: Chemistry and Properties

Polydentate ligands are most widely used in complexometric titrations because they form more stable complexes with the metal ions than mono- or bidentate ligands due to the chelate effect. Examples of polydentate ligands are ethylenediaminetetraacetic acid (EDTA), crown ethers, and cryptands. The most important feature of optimal polydentate ligands is the ability to form 1:1 complexes in a single-step process. Amino carboxylic acid derivatives are frequently used as complexing agents. EDTA is...

EDTA: Auxiliary Complexing Reagents

EDTA: Auxiliary Complexing Reagents

EDTA titrations are usually carried out in highly basic conditions, where the fully deprotonated form of EDTA, Y4−, actively complexes with the free metal ions in the solution. Several metal ions precipitate as hydrous oxide (hydroxides, oxides, or oxyhydroxides) under these conditions, lowering the concentration of free metal ions in the solution. For this reason, auxiliary complexing agents or ligands such as ammonia, tartrate, citrate, or triethanolamine are used in EDTA titrations to...

Venous Thrombosis III: Interprofessional Care

Venous Thrombosis III: Interprofessional Care

Venous thrombosis requires effective prevention and treatment strategies to improve patient outcomes and reduce potential complications.Prevention StrategiesHealthcare providers must prioritize preventing venous thromboembolism (VTE) for all adult patients upon admission. Interventions depend on bleeding and thrombosis risk, medical history, current medications, diagnoses, planned procedures, and patient preferences. Patients on bed rest should change positions every two hours and, if not...

Effects of EDTA on End-Point Detection Methods

Effects of EDTA on End-Point Detection Methods

Different methods, such as visual observance of metal-ion indicators, spectroscopic techniques, and potentiometric methods, can determine the endpoint of an EDTA titration.
In the visual method, metal-ion indicators (metallochromic dyes), which have distinct colors in their free and complex forms, are added to the mixture to signal the titration's end point. They form stable complexes with metal ions, but these complexes are weaker than the corresponding metal–EDTA complexes. As a result, EDTA...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Diagnosis of infections in newborns using a new particle-mediated immunoassay for serum C-reactive protein.

The Journal of automatic chemistry·2008

Same author

First basic performance evaluation of the XE-2100 haematology analyser.

Journal of automated methods & management in chemistry·2008

Same author

Automated bone marrow analysis using the CD4000 automated haematology analyser.

Journal of automated methods & management in chemistry·2008

Same author

Evaluation of use of the optional unit QA-810V for the determination of five-part leukocyte differentials.

Journal of automated methods & management in chemistry·2008

Same author

Toward an interpretation of dynamic neural activity in terms of chaotic dynamical systems.

The Behavioral and brain sciences·2002

Same author

Flow cytometric analysis of platelet activation under calcium ion-chelating conditions.

Clinical and laboratory haematology·2002

Same journal

A Novel Sandwich Electrochemical Immunosensor Based on the DNA-Derived Magnetic Nanochain Probes for Alpha-Fetoprotein.

Journal of automated methods & management in chemistry·2011

Same journal

Determination of low levels of lead in beer using solid-phase extraction and detection by flame atomic absorption spectrometry.

Journal of automated methods & management in chemistry·2011

Same journal

A LabVIEW-Based Virtual Instrument System for Laser-Induced Fluorescence Spectroscopy.

Journal of automated methods & management in chemistry·2011

Same journal

Synthesis and physical properties of estolide ester using saturated Fatty Acid and ricinoleic Acid.

Journal of automated methods & management in chemistry·2011

Same journal

Erratum: to "Development of a Low-Cost SIA-Based Analyser for Water Samples".

Journal of automated methods & management in chemistry·2011

Same journal

A simple automated method for the determination of nitrate and nitrite in infant formula and milk powder using sequential injection analysis.

Journal of automated methods & management in chemistry·2011

See all related articles

Search research articles

Related Experiment Video

Updated: Jun 28, 2026

In Vitro Thrombosis Test for Ventricular Assist Devices

In Vitro Thrombosis Test for Ventricular Assist Devices

Published on: March 21, 2025

CTAD as a universal anticoagulant.

M Yokota¹, N Tatsumi, I Tsuda

¹Department of Clinical and Laboratory Medicine, Osaka City University Medical School, 1-4-3 Ashinachi, Abeno, Osaka 545-8585, Japan.

Journal of Automated Methods & Management in Chemistry

|October 18, 2008

Summary

This summary is machine-generated.

CTAD, a new anticoagulant mixture, shows promising results for medical laboratory use. It provides results similar to EDTA and citrate for blood counts and coagulation tests, making it a viable option.

More Related Videos

Assessment of the Anticoagulant and Anti-inflammatory Properties of Endothelial Cells Using 3D Cell Culture and Non-anticoagulated Whole Blood

Assessment of the Anticoagulant and Anti-inflammatory Properties of Endothelial Cells Using 3D Cell Culture and Non-anticoagulated Whole Blood

Published on: September 5, 2017

The Nijmegen Hemostasis Assay: Simultaneous Fluorogenic Measurement of Thrombin and Plasmin Generation in a Single Well

The Nijmegen Hemostasis Assay: Simultaneous Fluorogenic Measurement of Thrombin and Plasmin Generation in a Single Well

Published on: February 27, 2026

Related Experiment Videos

Last Updated: Jun 28, 2026

In Vitro Thrombosis Test for Ventricular Assist Devices

In Vitro Thrombosis Test for Ventricular Assist Devices

Published on: March 21, 2025

Assessment of the Anticoagulant and Anti-inflammatory Properties of Endothelial Cells Using 3D Cell Culture and Non-anticoagulated Whole Blood

Assessment of the Anticoagulant and Anti-inflammatory Properties of Endothelial Cells Using 3D Cell Culture and Non-anticoagulated Whole Blood

Published on: September 5, 2017

The Nijmegen Hemostasis Assay: Simultaneous Fluorogenic Measurement of Thrombin and Plasmin Generation in a Single Well

The Nijmegen Hemostasis Assay: Simultaneous Fluorogenic Measurement of Thrombin and Plasmin Generation in a Single Well

Published on: February 27, 2026

Area of Science:

Clinical Chemistry
Hematology
Medical Laboratory Science

Background:

Anticoagulants are crucial for preserving blood sample integrity.
Existing anticoagulants like EDTA and citrate have limitations.
There is a need for novel anticoagulants in laboratory diagnostics.

Purpose of the Study:

To evaluate the feasibility of CTAD (citrate, theophylline, adenosine, dipyridamole) as a new anticoagulant.
To compare CTAD's performance against established anticoagulants (EDTA, citrate).
To assess CTAD's impact on various laboratory test parameters.

Main Methods:

Prospective study design.
Whole blood samples were anticoagulated with CTAD.
Comparison of complete blood counts, white cell differentials, plasma chemistry, and coagulation tests (PT, aPTT, fibrinogen) with EDTA and citrate anticoagulated samples.

Main Results:

CTAD demonstrated high similarity to EDTA for complete blood count and white cell differential.
Minor reductions in platelet count and mean platelet volume were observed with CTAD.
Plasma chemistry results from CTAD were comparable to serum.
Coagulation test results (PT, aPTT, fibrinogen) were similar to citrate plasma.

Conclusions:

CTAD is a feasible and effective anticoagulant for medical laboratory applications.
CTAD offers comparable performance to existing anticoagulants for a wide range of tests.
CTAD presents a promising alternative anticoagulant for laboratory diagnostics.