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Updated: Jun 28, 2026
Murine Colitis Modeling using Dextran Sulfate Sodium (DSS)
Published on: January 19, 2010
Amelioration of murine dextran sulfate sodium-induced colitis by nuclear factor-kappaB decoy oligonucleotides
Jun Ying Xiang1, Li Guo Wu, Xiao Li Huang
1Department of Gastroenterology, West China Hospital, Sichuan University, Sichuan, China.
Background:
Activation of nuclear factor (NF)-kappaB has been shown to play a critical role in the pathogenesis of ulcerative colitis (UC). The purpose of the current study was to investigate the effects of NF-kappaB decoy oligonucleotides (ODNs) on an experimental model of UC.
Methods:
NF-kappaB decoy ODNs were administered in experimental colitis induced by dextran sulfate sodium (DSS). The disease activity index (DAI) and histological score were observed. NF-kappaB DNA binding activity was assessed by electrophoretic mobility shift assay (EMSA). The expression of tumor necrosis factor-alpha (TNF-alpha) and interleukin-1beta (IL-1beta) were measured by reverse transcription polymerase chain reaction (RT-PCR) and enzyme-linked immunosorbent assay (ELISA).
Results:
A significant improvement was observed in DAI and histological score in mice with NF-kappaB decoy ODNs, and the increase in NF-kappaB DNA binding activity, myeloperoxidase (MPO) activity, IL-1beta, and TNF-a in mice with DSS-induced colitis was significantly reduced following administration of NF-kappaB decoy ODNs.
Conclusions:
The administration of NF-kappaB decoy ODNs leads to an amelioration of DSS-induced colitis, suggesting administration of NF-kappaB decoy ODNs may provide a therapeutic approach for UC.
Insights
NF-kappaB decoy oligonucleotides (ODNs) significantly improved experimental ulcerative colitis (UC) in mice. This suggests ODNs may offer a new therapeutic strategy for treating UC patients.
Area of Science:
- Gastroenterology
- Immunology
- Molecular Biology
Background:
- Nuclear factor-kappaB (NF-kappaB) activation is implicated in ulcerative colitis (UC) pathogenesis.
- Understanding NF-kappaB's role is crucial for developing targeted UC therapies.
Purpose of the Study:
- To investigate the therapeutic potential of NF-kappaB decoy oligonucleotides (ODNs) in an experimental model of UC.
Main Methods:
- Experimental colitis was induced using dextran sulfate sodium (DSS).
- NF-kappaB decoy ODNs were administered to assess their effects.
- Disease activity index (DAI), histological scores, NF-kappaB DNA binding activity, and inflammatory markers (TNF-alpha, IL-1beta) were evaluated.
Main Results:
- NF-kappaB decoy ODN administration significantly reduced DAI and histological scores in DSS-induced colitis.
- A notable decrease in NF-kappaB DNA binding activity, MPO activity, IL-1beta, and TNF-alpha was observed.
Conclusions:
- NF-kappaB decoy ODNs effectively ameliorated DSS-induced colitis in a preclinical model.
- These findings suggest NF-kappaB decoy ODNs hold promise as a novel therapeutic approach for ulcerative colitis.
