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Capillary Electrophoresis: Applications

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Capillary electrophoretic separations offer various modes, each with unique applications. These modes include capillary zone electrophoresis, capillary gel electrophoresis, capillary array electrophoresis, capillary isoelectric focusing, capillary isotachophoresis, micellar electrokinetic chromatography, and capillary electrochromatography.
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Updated: Jun 28, 2026

Sheathless Capillary Electrophoresis–Mass Spectrometry for Metabolic Profiling of Biological Samples

Sheathless Capillary Electrophoresis–Mass Spectrometry for Metabolic Profiling of Biological Samples

Published on: October 1, 2016

Micellar electrokinetic chromatography for high-performance analytical separation.

Shigeru Terabe¹

¹Graduate School of Material Science, University of Hyogo, 3-2-1 Kouto, Kamigori, Hyogo 678-1297, Japan. terabe@gaia.eonet.ne.jp

Chemical Record (New York, N.Y.)

|October 29, 2008

Summary

This summary is machine-generated.

Micellar electrokinetic chromatography (MEKC) enables the separation of neutral analytes, overcoming a key limitation of capillary electrophoresis (CE). This technique utilizes micelles to provide apparent charges for neutral molecules, enhancing analytical separation capabilities.

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Area of Science:

Analytical Chemistry
Separation Science

Background:

Capillary electrophoresis (CE) offers high efficiency, minimal sample requirements, low cost, and rapid separations.
Traditional CE separates analytes based on charge, limiting its application for neutral compounds.

Purpose of the Study:

To review the development and utility of micellar electrokinetic chromatography (MEKC).
To explain how MEKC overcomes the limitations of CE for neutral analyte separation.

Main Methods:

MEKC involves adding ionic micelles to the electrophoretic medium.
Neutral analytes partition into micelles, acquiring an apparent charge for separation.
The separation mechanism is analogous to chromatography, with micelles acting as a pseudo-stationary phase.

Main Results:

MEKC effectively separates neutral analytes by incorporating them into micelles.
The migration velocity of analytes is influenced by their interaction with the micelles.
Extensive theoretical studies have elucidated analyte behavior in MEKC.

Conclusions:

MEKC has become a popular and powerful separation mode within CE.
The technique significantly expands the applicability of electrophoretic separations to neutral compounds.