Related Experiment Video
Updated: Jun 28, 2026
05:16
UV-Vis Spectroscopic Characterization of Nanomaterials in Aqueous Media
Published on: October 25, 2021
Photometric titrations-XII A full-immersion spectrophotometer
1School of Chemistry, Georgia Institute of Technology, Atlanta, Georgia, U.S.A.
Talanta
|December 1, 1968
Summary
A novel photometric titration apparatus with variable path-length, zero suppression, and scale expansion was designed. This versatile instrument also functions as a simple spectrophotometer for enhanced analytical capabilities.
Area of Science:
- Analytical Chemistry
- Instrumental Analysis
- Photometry
Background:
- Photometric titrations are essential for quantitative chemical analysis.
- Existing instrumentation may lack flexibility in path-length and signal processing.
- The need for adaptable analytical tools is crucial in research and quality control.
Purpose of the Study:
- To design and describe a versatile photometric titration apparatus.
- To incorporate features for enhanced analytical performance, including variable path-length.
- To enable the instrument's dual use as a simple spectrophotometer.
Main Methods:
- Development of a photometric titration apparatus incorporating variable path-length.
- Integration of zero suppression and scale expansion functionalities.
- Testing of the apparatus for photometric titration and spectrophotometric measurements.
Main Results:
- A functional photometric titration apparatus was successfully designed.
- The apparatus demonstrated capabilities for variable path-length adjustments.
- The instrument proved effective for both titration and basic spectrophotometry.
Conclusions:
- The designed apparatus offers enhanced flexibility for photometric titrations.
- The dual functionality as a spectrophotometer increases its utility.
- This instrument provides a valuable tool for various analytical chemistry applications.
Related Concept Videos
01:14UV–Vis Spectrometers
The absorbance of UV and visible (UV–visible) radiations is measured using a UV–visible spectrophotometer. Deuterium lamps, which emit UV radiation, and tungsten lamps, which produce radiation in the visible region, are used as light sources in UV–visible spectrophotometers. A monochromator or prism is used for diffraction grating, i.e., to split the incoming radiation into different wavelengths. A system of slits is used to focus the desired wavelength on the sample cell. Samples for...
01:16Spectrophotometry: Introduction
Spectrophotometry is the quantitative measurement of the absorption, reflection, diffraction, or transmission of electromagnetic radiation through a material as a function of the intensity and wavelength of the radiation. A spectrophotometer is a device used to measure the change in the radiation intensity caused by its interaction with the material.
The essential components of a spectrophotometer include a source of electromagnetic radiation, a slot for placing a material to be analyzed, and a...
The essential components of a spectrophotometer include a source of electromagnetic radiation, a slot for placing a material to be analyzed, and a...
01:09UV–Vis Spectroscopy: Beer–Lambert Law
The Beer-Lambert law describes the relationship between absorbance and concentration, which combines the principles established by scientists Johann Heinrich Lambert and August Beer. Lambert's law states that when light passes through a medium, the loss in intensity is directly proportional to the original intensity and the path length of the light. Beer's law proposed that the transmittance of a solution remains constant if the product of concentration and path length is constant. The modern...
01:32UV–Vis Spectroscopy of Conjugated Systems
Organic compounds with conjugated double bonds show strong absorption features in the UV–visible region of the electromagnetic spectrum attributed to π → π* electronic excitations. Generally, a UV–vis absorption spectrum is recorded as a plot of absorbance vs wavelength. The wavelength of maximum absorbance, which manifests as a peak in the absorption spectrum, is denoted as λmax.
One of the factors influencing λmax is the extent of conjugation in the...
One of the factors influencing λmax is the extent of conjugation in the...
00:39Complexometric Titration: Overview
Complexometric titration involves the formation of a complex by reacting a metal ion with one or more ligands. A visual indicator often detects the end point of a complexometric titration. It is added to the metal solution before the titration, forming a stable metal–indicator complex and imparting color to the solution. As the titration approaches the equivalence point, the excess of the added ligand displaces the indicator from the metal–indicator complex, releasing the free indicator. The...
01:05Total Internal Reflection Fluorescence Microscopy
Total internal reflection fluorescence microscopy or TIRF is an advanced microscopic technique used to visualize fluorophores in samples close to a solid surface with a higher refractive index, such as a glass coverslip. TIRF only allows fluorophores in proximity to the solid surface to be excited. When light from a medium with a lower refractive index (such as air) hits the glass coverslip at a critical angle, the light undergoes total internal reflection stead of passing through the glass.