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Updated: Jun 28, 2026

High-throughput Fluorometric Measurement of Potential Soil Extracellular Enzyme Activities

High-throughput Fluorometric Measurement of Potential Soil Extracellular Enzyme Activities

Published on: November 15, 2013

[Not Available].

G Ghimicesuc¹, V Dorneanu

¹Institut de Médecine et de Pharmacie de Iassy, Roumanie.

|March 1, 1972

Summary

This summary is machine-generated.

This study presents a simple colorimetric method for determining phosphate levels in biological, pharmaceutical, and food samples. The assay offers high sensitivity and accuracy for phosphate analysis.

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Area of Science:

Analytical Chemistry
Spectrophotometry
Inorganic Chemistry

Context:

Accurate phosphate determination is crucial in various fields, including environmental monitoring, clinical diagnostics, and food quality control.
Existing methods for phosphate analysis may be complex, time-consuming, or require specialized equipment.
There is a need for a sensitive, accurate, and widely applicable method for phosphate quantification.

Purpose:

To develop and validate a simple colorimetric micromethod for the quantitative determination of phosphates.
To establish the sensitivity, accuracy, and applicability of the proposed method across diverse sample matrices.
To provide a reliable analytical tool for phosphate analysis in biological substances, pharmaceuticals, and food products.

Summary:

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Last Updated: Jun 28, 2026

High-throughput Fluorometric Measurement of Potential Soil Extracellular Enzyme Activities

High-throughput Fluorometric Measurement of Potential Soil Extracellular Enzyme Activities

Published on: November 15, 2013

A novel colorimetric micromethod for phosphate determination is proposed, utilizing the precipitation of magnesium uranyl phosphate.
The precipitated compound is dissolved, and the uranyl ion is quantified spectrophotometrically by measuring the absorbance of the dark red uranyl ferrocyanide complex.
The method demonstrates a sensitivity of 0.01 mg of phosphorus (P) with an average error of 1%.

Impact:

This method offers a highly sensitive and accurate approach for phosphate analysis, suitable for routine laboratory use.
Its simplicity and broad applicability make it a valuable tool for quality control and research in various scientific disciplines.
The assay provides a cost-effective and efficient alternative for phosphate determination in diverse sample types.