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1Faculté de Pharmacie, Laboratoire de Chimie Analytique et Bromatologie, Domaine de la Merci, 38700 La Trouche, France.
Talanta
|December 1, 1986
Summary
High-performance liquid chromatography (HPLC) effectively quantifies natural xanthines like caffeine in beverages. This method is suitable for complex matrices such as coffee and tea extracts.
Area of Science:
- Analytical Chemistry
- Pharmacology
Context:
- Xanthines, including caffeine, theobromine, and theophylline, are naturally occurring alkaloids found in various beverages.
- Accurate quantification of these compounds is essential for quality control and understanding their physiological effects.
Purpose:
- To determine the three natural xanthines (caffeine, theobromine, theophylline) using High-Performance Liquid Chromatography (HPLC).
- To validate HPLC as a suitable method for analyzing xanthines in complex matrices like beverages.
Summary:
- HPLC analysis was performed using silica gel or C(18)-bonded phase columns.
- For beverage analysis, HPLC on silica gel proved effective for quantifying xanthines in coffee, tea, and maté, both in extracts and as consumed.
- The HPLC method demonstrated suitability for complex sample matrices, with comparisons made to other caffeine determination techniques.
Impact:
- Establishes HPLC as a robust analytical technique for xanthine determination in diverse and complex food and beverage samples.
- Provides a reliable method for quality control and research involving natural xanthines.
