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Spectrophotometric determination of tetracyclines in pharmaceutical preparations, with uranyl acetate

U Saha1, A K Sen, T K Das

  • 1Central Public Health & Drug Laboratory, 2 Convent Lane, Calcutta 700016, India.

Talanta
|December 1, 1990
PubMed

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The absorbance of UV and visible (UV–visible) radiations is measured using a UV–visible spectrophotometer. Deuterium lamps, which emit UV radiation, and tungsten lamps, which produce radiation in the visible region, are used as light sources in UV–visible spectrophotometers. A monochromator or prism is used for diffraction grating, i.e., to split the incoming radiation into different wavelengths. A system of slits is used to focus the desired wavelength on the sample cell. Samples for...